雪胆素甲对胃癌细胞SGC-7901增殖和侵袭能力的影响  

EFFECT OF CUCURBITACINⅡA ON THE PROLIFERATION AND INVASION OF GASTRIC CANCER SGC-7901 CELLS

作　　者：曹淑君 初向华[1] 李宗莉[1] 张翠萍[2] 孙向红[1] CAO Shujun;CHU Xianghua;LI Zongli;ZHANG Cuiping;SUN Xianghong(Department of Pharmacy, The Affilicated Hospital of Qingdao University, Qingdao 266003, China)

机构地区：[1]青岛大学附属医院药剂科,山东青岛2660031 [2]青岛大学附属医院消化内科,山东青岛2660031

出　　处：《精准医学杂志》2020年第6期540-544,547,共6页Journal of Precision Medicine

基　　金：山东省自然科学基金资助项目(ZR2014HM094)。

摘　　要：目的研究雪胆素甲(CuⅡa)对胃癌SGC-7901细胞增殖和迁移能力的影响及其分子作用机制。方法以0、0.01、0.1、1、10、100μmol/L的CuⅡa溶液(A、B、C、D、E、F组)作用SGC-7901细胞48 h后,采用CCK-8法、Transwell小室、流式细胞仪分别检测细胞的增殖能力、迁移能力及细胞凋亡情况。采用免疫印迹(Western blot)法分析细胞中β-连环蛋白(β-catenin)、C-myc、细胞周期素D1(CyclinD1)和糖原合成酶激酶3β(GSK-3β)等蛋白的表达。结果除B组细胞的迁移能力以及凋亡率与A组相比,差异无显著性(P>0.05)外,其他组与A组相比,随着CuⅡa浓度的增加,可以明显地抑制SGC-7901细胞的增殖、迁移及促进SGC-7901细胞的凋亡(F=15.812~98.346,P<0.05),且其作用呈浓度依赖性。与A组相比,0.1、1、10、100μmol/L的CuⅡa作用48 h后,可以降低胃癌细胞β-catenin、C-myc、CyclinD1蛋白的表达(F=15.632、8.494、11.267,P<0.05),增加GSK-3β蛋白的表达(F=21.763,P<0.05),但是B组β-catenin、C-myc、CyclinD1、GSK-3β蛋白表达与A组相比,差异无显著的统计学意义(P>0.05)。结论CuⅡa能够有效地抑制胃癌细胞的增殖和侵袭,诱导胃癌细胞凋亡,其机制可能与抑制Wnt/β-catenin信号通路的激活有关。Objective\To investigate the effect of cucurbitacinⅡa(CuⅡa)on the proliferation and migration of gastric cancer SGC-7901 cells and related molecular mechanism.\Methods\SGC-7901 cells were treated with CuⅡa solution at a concentration of 0,0.01,0.1,1,10,and 100μmol/L(groups A,B,C,D,E,and F,respectively)for 48 h,and then the CCK-8 assay,Transwell chamber,and flow cytometry were used to observe the proliferation,migration,and apoptosis of SGC-7901 cells.Wes-tern blot was used to measure the protein expression ofβ-catenin,C-myc,cyclinD1,and glycogen synthase kinase-3β(GSK-3β)in cells.\Results\There were no significant differences in the migration ability and apoptotic rate of SGC-7901 cells between groups B and A(P>0.05),while compared with group A,groups C,D,E,and F showed significant inhibition of the proliferation and migration of SGC-7901 cells and significant promotion of the apoptosis of SGC-7901 cells(F=15.812-98.346,P<0.05)in a concentration-dependent manner.Compared with group A,groups C,D,E,and F had significant reductions in the protein expression ofβ-catenin,C-myc,and cyclinD1(F=15.632,8.494,11.267,P<0.05)and a significant increase in the protein expression of GSK-3β(F=21.763,P<0.05)after 48-hour treatment with CuⅡa solution at a concentration of 0.1,1,10,and 100μmol/L.There were no significant differences in the protein expression ofβ-catenin,C-myc,cyclinD1,and GSK-3βbetween groups B and A(P>0.05).\Conclusion\CuⅡa can effectively inhibit the proliferation and invasion of gastric cancer cells and induce apoptosis of gastric cancer cells,possibly by inhibiting activation of the Wnt/β-catenin signaling pathway.

关 键 词：雪胆素甲 胃肿瘤 细胞增殖 细胞凋亡 肿瘤侵润 WNT信号通路 

分 类 号：R735.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]