半枝莲多糖对宫颈癌荷瘤小鼠抑瘤作用的实验研究  

The tumor-inhibiting effect of SBP on cervical cancer in mice

作　　者：袁辉[1] 孙健[1] 徐吉雨 许晓义[1] 徐文秀[1] 李丽[1] 石屹[1] Yuan Hui

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中医临床研究》2020年第32期5-7,共3页Clinical Journal Of Chinese Medicine

基　　金：2018年度黑龙江省省属高等学校基本科研业务费科研项目(No.2018-KYYWFMY-0043);牡丹江市科学技术计划项目(No.z2018s040)。

摘　　要：目的:观察半枝莲多糖(Sculellaria Barbata polysaccharides,SBP)对宫颈癌荷瘤小鼠血清中血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)、白细胞介素-2(Interleukin-2,IL-2)、基质金属蛋白酶(Matrix Metalloproteinase,MMP)2和MMP9含量及瘤组织中兔抗人单克隆抗体(Bax)、B淋巴细胞瘤-2(Bcl-2)、半胱氨酸蛋白酶(Caspase)-3和Caspase-9基因表达的影响,探讨其抗肿瘤的机制。方法:50只裸鼠于右腋下部接种瘤细胞悬液制作宫颈癌荷瘤小鼠模型,随机分成模型对照组、半枝莲多糖高、中、低剂量组、环磷酰胺(CTX)组,酶联免疫吸附剂测定(Enzyml Linded Immunosorbe Assay,ELISA)法检测各组鼠血清中VEGF、IL-2、MMP2和MMP9的含量变化;qRT-PCR检测Bcl-2、Bax、Caspase-3和Caspase-9基因表达情况。结果:不同剂量的半枝莲多糖能够增加脾脏指数,抑制肿瘤生长;可减少鼠血清中VEGF、MMP2和MMP9的含量,增加IL-2的含量;可增加Bax、Caspase-3和Caspase-9基因表达,减少Bcl-2基因表达,以中剂量组作用最显著。结论:半枝莲多糖可通过调节上述相关因子,抑制宫颈癌荷瘤小鼠肿瘤的进展。Objective:To observe the effects of SBP on gene expression of VEGF,IL-2,MMP2,MMP9,Bax,Bcl-2,Caspase-3 and Caspase-9 of cervical cancer-bearing mice,to explore its anti-tumor mechanism.Methods:Cervical cancer tumor-bearing mouse model established by inoculated tumor cell suspension into the right axilla,50 nude mice were randomly divided into the model control group,SBP high dose group,SBP medium dose group,SBP low dose group,CTX group.VEGF,IL-2,MMP2 and MMP9 were detected by ELISA.Bax,Bcl-2,Caspase-3 and Caspase-9 were detected by qRT-PCR.Results:SBP of different doses can increase spleen index,inhibit tumor growth,reduce the content of serum VEGF,MMP2 and MMP9,increase IL-2,increase the genes of Bax,Caspase-3 and Caspase-9 and reduce the gene expression of Bcl-2,and the SBP middle dose group shows most significant effect.Conclusion:SBP can inhibit the tumor progression of cervical cancer tumor-bearing mice by regulating the above-mentioned related factors.

关 键 词：半枝莲多糖 宫颈癌 血管内皮生长因子 基质金属蛋白酶2 B淋巴细胞瘤-2 

分 类 号：R73[医药卫生—肿瘤]