胶束介导联合超声—微波辅助法提取喜树叶中喜树碱及羟基喜树碱  

Extraction of Camptothecin and Hydroxyl Camptothecin from Camptothecin Leaves by Micellar Mediation Combined with Ultrasound-Microwave-Assisted Method

作　　者：杨建航 赵修华[1] YANG Jian-Hang;ZHAO Xiu-Hua(College of Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学化学化工与资源利用学院,哈尔滨150040

出　　处：《植物研究》2020年第6期951-960,共10页Bulletin of Botanical Research

基　　金：国家重点研发计划项目(2017YFD060070601)。

摘　　要：为了寻找一种新的方法从喜树叶中提取喜树碱和羟基喜树碱,充分利用喜树资源,以喜树叶粉末作为提取原料,采用超声—微波辅助提取技术提取喜树叶中活性成分喜树碱和羟基喜树碱。采用响应面优化方法设计,分别考察微波时间、料液比、微波功率、表面活性剂的浓度4个因素对总提取率的影响,优化得到最佳提取工艺条件。研究结果表明:当微波时间为10.5 min、料液比为1∶60、微波功率为850 W、表面活性剂浓度为8.5 g·L^-1时总提取率达到最佳。最佳工艺下总提取率为0.0564%。In order to explore a new method to extract camptothecin and hydroxy-camptothecin from camptothecin leaves and make full use of camptothecin resources,the active ingredients of camptothecin and hydroxy-camptothecin from camptothecin leaves were extracted from camptothecin leaves powder as raw material.In this paper,the influence of microwave processing time,solid-liquid ratio,microwave power and the concentration of surfactant on the total extraction rate were investigated respectively,and the optimum extraction conditions were obtained.The results showed that the optimal extraction rate was achieved when the microwave processing time was 10 min 30 sec,the solid-liquid ratio was 1∶60,the microwave power was 850 W,and the concentration of surfactant was 8.5 g·L^-1.The total extraction rate was 0.0564%under the optimal process.

关 键 词：喜树碱 羟基喜树碱 表面活性剂 超声—微波辅助法 

分 类 号：R914.4[医药卫生—药物化学]