高压蒸汽灭菌后的内镜注水瓶及其治疗用水的安全使用时间  

The safe service time of endoscopic water injector and its therapeutic water after autoclaving

作　　者：周庆[1] 徐杰[1] 王鸣[1] 张钰[1] 王志强[1] Zhou Qing;Xu Jie;Wang Ming(Department of Gastroenterology,the Second Medical Center,Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]解放军总医院第二医学中心消化内科,北京100853

出　　处：《中华保健医学杂志》2020年第6期621-623,共3页Chinese Journal of Health Care and Medicine

基　　金：解放军总医院医疗大数据研发项目(2017MBD-023)。

摘　　要：目的根据胃肠镜注水瓶高压蒸汽灭菌后微生物污染情况,研究注水瓶治疗用水的安全使用时间。方法按照注水瓶治疗用水的盛注方式和采样部位分组,模拟胃肠镜检查对注水瓶的治疗用水分2种盛注方式、2个采样部位进行5个周期采样,样本收集于无菌培养瓶内立即接种于哥伦比亚血培养皿培养,观察菌落计数。结果注水瓶经高压蒸汽灭菌后每日更换水组72个样本培养35 d均无细菌生长,合格率100%。每日添加水组72个样本在第4、5个周期共检出6个不合格样本。更换水组和添加水组合格率差异无统计学意义(P=0.077),不同时间周期的采样合格率差异无统计学意义(P=0.177)。结论在标准化无菌操作且保证注水瓶内为灭菌注射用水的条件限制下,注水瓶间隔7 d进行高压蒸汽灭菌,瓶内治疗用水在本实验微生物感染监测结果中显示是安全的。Objective To study the safe use time of treatment water for endoscopy water bottle after autoclaved based on the microbial contamination.Methods The samples were grouped according to the filling method and sampling site of the endoscopy water bottle.Treatment water was collected from endoscopy water bottle by simulating gastroscopy in two injection modes and two sampling sites for five cycles.The samples were collected in sterile culture bottles and immediately inoculated into Columbia blood culture dishes to observe the colony count.Results After high-pressure steam sterilization,72 samples of the water group which were replaced daily were detected without bacterial growth for 35 days.The qualification rate was 100%.A totals of 6 unqualified samples were detected in the fourth and fifth cycles of 72 samples in the daily water addition group.There was no significant difference between the replacement water group and the added water group(P=0.077),same as in the sampling qualification rate at different time periods(P=0.177).Conclusion Under the conditions of standardized aseptic operation,sterilized water for injection,and high-pressure steam sterilization of the endoscopy water bottle in one week apart,the treatment water in the endoscopy water bottle is shown to be safe in the experimental microbial infection monitoring.

关 键 词：内镜注水瓶 高压灭菌 安全 使用周期 

分 类 号：R573[医药卫生—消化系统]