桑色素-Cu^2+配位分子印迹聚合物制备及其固相萃取应用  

Preparation of morin-Cu complex imprinted polymer and its application in solid phase extraction

作　　者：邢占芬 成洪达 张平平 王清路 XING Zhan-fen;CHENG Hong-da;ZHANG Ping-ping;WANG Qing-lu(Department of Phamiacy,Qilu Medical University,Zibo 255300,China;Key Laboratory of Biomedical Engineering and Technology,Qilu Medical University,Zibo 255300,China)

机构地区：[1]齐鲁医药学院药学院,山东淄博255300 [2]齐鲁医药学院生物医学工程技术重点实验室,山东淄博255300

出　　处：《中草药》2020年第23期5943-5948,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31701042);淄博市校城融合发展计划项目(2018ZBXC403);淄博市校城融合发展计划项目开放课题(2019GECA018);淄博市校城融合发展计划项目开放课题(2019GECA019)。

摘　　要：目的制备桑色素-Cu^2+配位分子印迹聚合物,对桑枝中桑色素进行分离富集。方法在Cu^2+存在下,丙烯酰胺为功能单体,在水-甲醇极性溶剂中制备桑色素-Cu^2+配位分子印迹聚合物,以该聚合物为填料固相萃取桑枝中桑色素。结果所制备配位分子印迹聚合物对桑色素具有较强的特异性、选择性吸附,最大吸附量82μmol/g,远大于以氢键为作用力的传统印迹聚合物及非印迹聚合物;对桑色素结构类似物大豆素、儿茶素的分离因子分别为4.81、4.02。对比桑枝固相萃取淋洗液、洗脱液组成,所制备配位印迹聚合物对桑枝中桑色素表现出明显的富集效果。结论制备的桑色素配位分子印迹聚合物环境适应性好,选择性吸附能力强,是适用于天然产物有效成分桑色素分离富集的良好材料。Objective To prepare morin-Cu^2+ complex imprinted polymer(CIP) and use the polymer to extract morin from mulberry branch. Methods Morin-Cu^2+ CIPs were prepared in water-methanol polar solvent in the presence of Cu^2+, acrylamide as the functional monomer. The CIPs were used as the solid phase extraction sorbent for extracting morin from mulberry branch. Results The prepared CIPs had specific and selective adsorption on morin, the maximum adsorption capacity was 82 μmol/g, which was much higher than that of the traditional imprinted polymers relying on hydrogen bond and non-imprinted polymers. The separation factors of CIPs to daidzein and catechin were 4.81 and 4.02, respectively. CIPs had a significant enrichment effect on morin in the composition of the mulberry branch solid phase extraction elution solution. Conclusion Morin-Cu^2+ CIPs are excellent material used for the separation and enrichment of morin, the material has good selective adsorption ability and environmental adaptability.

关 键 词：分子印迹技术 配位印迹聚合物 桑色素 固相萃取 CU2+ 丙烯酰胺 

分 类 号：R283.6[医药卫生—中药学]