1,8-桉叶素型互叶白千层挥发性成分提取与分析  

Extraction and Determination of Volatile Constituents of Melaleuca alternifoliaof 1,8-Cineole Type

作　　者：陈海燕[1] 李桂珍[1] 秦荣秀[1] 梁忠云[1] Chen Haiyan;Li Guizhen;Qin Rongxiu;Liang Zhongyun(Guangxi Forestry Research Institute,Guangxi Key Laboratory of Special Non-wood Forest Cultivation&Utilization,Nanning,Guangxi 530002,China)

机构地区：[1]广西壮族自治区林业科学研究院广西特色经济林培育与利用重点实验室,广西南宁530002

出　　处：《广西林业科学》2020年第4期604-608,共5页Guangxi Forestry Science

基　　金：广西特色经济林培育与利用重点实验室自主课题(19-A-04-02);广西科技基地和人才专项(桂科AD18281083);广西林科院基本科研业务费专项(林科201821号);广西林业科研与推广项目(gl2018kt15)。

摘　　要：采用水蒸气蒸馏法从1,8-桉叶素型互叶白千层中提取挥发油中的油相成分,用乙醚从蒸馏馏出液中萃取挥发油中的水溶性物质;利用气相色谱-质谱联用技术(GC-MS)对二者的化学成分进行定性分析,根据峰面积归一化法进行定量分析。结果表明,油相部分的得油率为2.66%(以鲜重计),确定了其中的26种成分,占油相成分总量的98.301%,其主要成分为1,8-桉叶素(68.290%,)、α-松油醇(9.980%)、苧烯(8.469%)、α-蒎烯(3.529%)、β-月桂烯(1.946%)和4-松油醇(1.416%)。水溶性成分得油率为0.0101%(以鲜重计),确定了其中的23种成分,占水相成分总量的97.205%,主要成分为1,8-桉叶素(45.363%)、α-松油醇(33.392%)、对二氧己环-2,3-二醇(13.465%)和4-松油醇(3.225%)。油相和水相中相同的组分有10个,其中3个主要的共同组分为1,8-桉叶素、α-松油醇和4-松油醇。Oil fraction of volatile components in volatile oil of Melaleuca alternifolia of 1,8-cineole type was obtained through steam distillation.Water-siluble fraction in volatile oil was obtained from distillate by aether.Chemical components were analyzed by GC-MS and peak area normolization method.Results showed that oil yields in oil fraction and water-siluble fraction were 2.66%and 0.0101%(both on fresh weight basis),respectively.26 com⁃pounds were identified in oil fraction,which accounting for 98.301%of oil fraction.23 compounds were identified in water-siluble fraction,which accouting for 97.205%of water-siluble fraction.Major compounds in oil fraction were 1,8-cineole(68.290%),α-terpineol(9.980%),D-limonene(8.469%),α-pinene(3.529%),β-myrcene(1.946%)and 4-terpineol(1.416%).Major compounds in water-siluble fraction were 1,8-cineole(45.363%),α-terpineol(33.392%),p-dioxane-2,3-diol(13.465%)and 4-terpineol(3.225%).There were 10 same compounds,including three major compounds of 1,8-cineole,α-terpineol and 4-terpineol.

关 键 词：挥发油 气相色谱-质谱联用技术 互叶白千层 1 8-桉叶素 

分 类 号：TQ654[化学工程—精细化工]