结直肠癌肝转移患者癌细胞白细胞介素-13受体α2的表达及其与骨髓源性抑制细胞的关系  

Expression of interleukin-13 receptorα2 in cancer cells of colorectal cancer patients with liver metastasis and its relationship with myeloid-derived suppressor cells

作　　者：余姝彦 杨逸 胡婷 曹艳佩 YU Shuyan;YANG Yi;HU Ting;CAO Yanpei(Department of General Surgery,Huashan Hospital Affiliated to Fudan University,Shanghai,200041;Nursing Department,Huashan Hospital Affiliated to Fudan University,Shanghai,200041)

机构地区：[1]复旦大学附属华山医院普外科,上海200041 [2]复旦大学附属华山医院护理部,上海200041

出　　处：《实用临床医药杂志》2020年第23期65-69,共5页Journal of Clinical Medicine in Practice

基　　金：国家自然科学基金面上项目(81472677)。

摘　　要：目的探讨白细胞介素-13受体α2(IL-13Rα2)在结直肠癌肝转移患者细胞中的表达及其与骨髓源性抑制细胞(MDSCs)的关系。方法回顾性分析2000—2013年复旦大学附属华山医院手术的68例结直肠癌肿瘤患者石蜡切片,使用常规免疫染色方案,切片观察IL-13Rα2表达。采用抗CD11B抗体和抗CD33抗体进行双重染色,采用荧光抗体、山羊抗兔免疫球蛋白(H+L)抗体和山羊抗鼠免疫球蛋白(H+L)抗体对CD33进行显示。3位病理专家进行双盲独立评估IL-13Rα2染色载玻片。结果结直肠癌肝转移患者IL-13Rα2阳性率最高(90.8%),其次为脑转移(65.0%)、原发病灶(60.0%)、其他转移(53.0%),差异有统计学意义(P<0.05)。在肝转移病例中,IL-13Rα2表达与MDSCs数量呈线性相关(r=0.740,P<0.05)。48例远处转移病例的平均生存期为(12.6±5.2)个月。结论IL-13Rα2水平升高提示结直肠癌远处转移患者的预后较差,推测IL-13Rα2通过调节结直肠癌细胞来诱导MDSCs增殖并促进肿瘤肝转移。Objective To investigate the expression of interleukin-13 receptorα2(IL-13Rα2)in cells of colorectal cancer patients with liver metastasis and its relationship with myeloid-derived suppressor cells(MDSCs).Methods Paraffin sections of 68 patients with colorectal cancer surgery in Huashan Hospital Affiliated to Fudan University from 2000 to 2013 were retrospectively analyzed.The expression of IL-13Rα2 was observed after routine immunostaining.Anti-CD11B antibody and anti-CD33 antibody were used for double staining.Fluorescent antibody,goat anti-rabbit immunoglobulin(H+L)antibody and goat anti-mouse immunoglobulin(H+L)antibody were used to display CD33.Three pathologists independently evaluated IL-13Rα2 stained slides in a double-blind manner.Results The positive rate of IL-13Rα2 in colorectal cancer patients with liver metastasis was the highest(90.8%),followed by brain metastasis(65.0%),primary lesions(60.0%),and other metastases(53.0%),and there were significant differences(P<0.05).There was a linear correlation between the expression of IL-13Rα2 and the number of MDSCs in cases with liver metastasis(r=0.740,P<0.05).The average survival time of 48 patients with distant metastasis was(12.6±5.2)months.Conclusion The increased level of IL-13Rα2 suggests that the prognosis of colorectal cancer patients with distant metastasis is poor,and it is speculated that IL-13Rα2 can induce proliferation of MDSCs and promote liver metastasis by regulating colorectal cancer cells.

关 键 词：白细胞介素-13受体 骨髓源性抑制细胞 结直肠癌 肝转移 脑转移 

分 类 号：R735.3[医药卫生—肿瘤] R329.2[医药卫生—临床医学]