Effect specificity of the early intervention with moxibustion at“BL23”in Alzheimer’s disease rats modeled by ovariectomy and D-Galactose injection  

艾灸“肾俞”早期干预去卵巢合D-半乳糖注射AD样大鼠效应中的特异性

作　　者：Yan-jun DU Yi-ming TAO Qing TIAN Peng ZENG Wen-hui WU Jia-huan XIAO 杜艳军;陶一鸣;田青;曾鹏;吴文辉;肖佳欢(湖北中医药大学针灸骨伤学院,武汉430061;华中科技大学同济医学院,武汉430000;武汉大学中南医院,武汉430000)

机构地区：[1]College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,China [2]Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430000,China [3]Zhongnan Hospital of Wuhan University,Wuhan 430000,China

出　　处：《World Journal of Acupuncture-Moxibustion》2020年第4期277-284,共8页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China Project:No.81473786;No.81873380。

摘　　要：Objective:To observe the effect of the early intervention with moxibustion at "Shènshū(肾俞BL23)" on learning and memory of Alzheimer’s disease(AD) rats modeled by ovariectomy and D-Galactose(D-Gal)injection and the effect on Ca2+/CaMKII/CREB signaling pathway so as to provide the evidence for the acupoint selection in the early intervention with acupuncture and moxibustion for AD.Methods:Sixty female SD rats at the age of 3 months were selected and randomly divided into a normal group,a sham-operation group,a model group,a "BL23" group,a "Wèishū"(胃俞BL21) group,and a non-acupoint group,10 rats in each group.Except in the normal group and the sham-operation group,bilateral ovaries were removed in the other 4 groups.3 days after the operation,D-Gal was injected intraperitoneally at a dose of 150 mg/kg/d for 90 days to establish AD rat model.In the sham-operation group,only the peripheral fat of ovarian was removed and 0.9% normal saline was injected intraperitoneally of the same dose for 90 days.In the "BL23" group,the "BL21" group and the non-acupoints group,since the 2 nd day after ovariectomy,moxibustion was applied at 9:00 a.m.every day in the rats.The rats were fixed on a fixed table,the mild moxibustion with self-prepared moxa stick was applied to bilateral "BL23""BL21" and the non-acupoint of each rat,the ignited end of moxa stick was 2 cm to 3 cm far from skin surface.The infrared thermometer was adopted to measure the temperature of the epidermis of each acupoint from time to time so as to maintain the local temperature to be(41 ± 0.5)℃.Mild moxibustion lasted for 10 min each time,consecutively for 5 days a week and at an interval of 2 days.The total consecutive intervention duration was 12 weeks.In the normal group and the shamoperation group,no moxibustion intervention was performed,but the rats were restricted in a same way during the experiment.At the end of modeling and intervention,water maze test was adopted to detect the learning and memory abilities of rats in each group.The western

关 键 词：Alzheimer's disease(AD) Ca^2+/caMKII/CREB Learning and memory ability MOXIBUSTION OVARIECTOMY “Shènshū(肾俞BL23)” 

分 类 号：R245.8[医药卫生—针灸推拿学]