Zn(Ⅱ)配位脂质体用于高效且血清耐受的基因转染  

Zinc(Ⅱ)coordinated lipid for effective and serum-tolerant gene delivery

作　　者：周晓红 Elbayomi Smaher M 聂旋 邵琪[1] 王海利 王飞 尤业字 Zhou Xiaohong;Elbayomi Smaher M;Nie Xuan;Shao Qi;Wang Haili;Wang Fei;You Yezi(CAS Key Laboratory of Soft Matter Chemistry,Department of Polymer Science and Engineering,University of Science and Technology of China,Hefei 230026,China;Neurosurgical Department,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230036,China)

机构地区：[1]中国科学院软物质化学重点实验室,中国科学技术大学高分子科学与工程系,安徽合肥230026 [2]中国科学技术大学附属第一医院神经外科,中国科学技术大学生命科学与医学部,安徽合肥230036

出　　处：《中国科学技术大学学报》2020年第11期1394-1402,共9页JUSTC

基　　金：the National Natural Science Foundation of China(51273187,21374107,21474097,52073269).

摘　　要：近年来,阳离子聚合物、阳离子脂质体等非病毒载体在基因递送中的应用越来越受到人们的关注.然而,血清的存在会降低它们的转染效率,从而阻碍它们在临床中的使用.因此,非常有必要开发能抵抗血清干扰的非病毒基因载体.本工作合成了一种新型的锌离子配位的脂质,其与1,2-二油酰-SN-甘油-3-磷酰乙醇胺脂质(DOPE)共自组装形成阳离子脂质体(Zn-DTAc脂质体).实验结果表明,Zn-DTAc脂质体可以有效地压缩质粒形成纳米复合物.即使在50%胎牛血清存在的情况下,该复合物也能进行有效的细胞内化和内涵体逃逸,并实现高效的基因转染.In recent years,non-viral vectors,such as cationic polymers and liposomes,have attracted increasing attentions for their applications in gene delivery.However,reduced transfection efficiency inhibits their further use in the presence of serum.Therefore,it is necessary to develop serum-tolerant non-viral gene vectors.Herein,a novel Zn coordinated lipid is synthesized,which self-assembles with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipid(DOPE),forming cationic liposome(Zn-DTAc liposome).The experimental results demonstrate that Zn-DTAc liposomes can effectively condense pDNA into nanoparticles,which have high cellular uptake and effective endosomal escape and exhibit efficient gene transfection even in the presence of 50%fetal bovine serum.

关 键 词：基因递送 非病毒载体 Zn(Ⅱ)配位脂质体 抗血清 

分 类 号：R318[医药卫生—生物医学工程]