Integrative analysis of the metabolome and transcriptome reveals seed germination mechanism in Punica granatum L.  

作　　者：FU Fang-fang PENG Ying-shu WANG Gui-bin Yousry A.EL-KASSABY CAO Fu-liang 

机构地区：[1]Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,P.R.China [2]Department of Forest and Conservation Sciences,Faculty of Forestry,The University of British Columbia,Vancouver,B.C.,V6T 1Z4,Canada

出　　处：《Journal of Integrative Agriculture》2021年第1期132-146,共15页农业科学学报（英文版）

基　　金：supported by the Doctorate Fellowship Foundation of Nanjing Forestry University, China (163010550);the Priority Academic Program Development of Jiangsu High Education Institutions, China (PAPD)。

摘　　要：We conducted an integrative system biology of metabolome and transcriptome profile analyses during pomegranate(Punica granatum L.) seed germination and utilized a weighted gene co-expression network analysis(WGCNA) to describe the functionality and complexity of the physiological and morphogenetic processes as well as gene expression and metabolic differences during seed germination stages. In total, 489 metabolites were detected, including 40 differentially accumulated metabolites. The transcriptomic analysis showed the expression of 6 984 genes changed significantly throughout the whole germination process. Using WGCNA, we identified modules related to the various seed germination stages and hub genes. In the initial imbibition stage(stage 1), the pivotal genes involved in RNA transduction and the glycolytic pathway were most active, while in the sprouting stage(stage 4), the pivotal genes were involved in multiple metabolic pathways. In terms of secondary metabolic pathways, we found flavonoid 4-reductase genes of anthocyanin biosynthesis pathway are most significantly affected during pomegranate seed germination, while the flavonol synthase gene was mainly involved in the regulation of isoflavonoid biosynthesis.

关 键 词：seed germination stages weighted gene co-expression network analysis(WGCNA) METABOLOME TRANSCRIPTOME flavonoid pathway 

分 类 号：S665.4[农业科学—果树学]