亚硒酸钠和硒代蛋氨酸对人结肠腺癌细胞的毒性作用  

Cytotoxicity in Human Colon Adenocarcinoma Caco-2 Cell Induced by Selenite and Selenomethionine

作　　者：覃焱 许海钊 徐境懋 顾明华[1] 韦燕燕[1] QIN Yan;XU Hai-zhao;XU Jing-mao;GU Ming-hua;WEI Yan-yan(Colleges of Guangxi Key Laboratory of Agricultural Environment and Agricultural Products Quality and Safety,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学农学院,广西农业环境与农产品质量安全重点实验室,广西南宁530004

出　　处：《现代食品科技》2021年第1期1-6,72,共7页Modern Food Science and Technology

基　　金：国家自然科学基金项目(41967048);广西自然科学基金项目(2016GXNSFAA380308);广西创新驱动重大专项(AA17202038)。

摘　　要：探讨两种硒化物对人结肠腺癌Caco-2细胞的毒性作用。将Caco-2细胞暴露在不同浓度的亚硒酸钠(SeIV)和硒代蛋氨酸(SeMet)中培养24 h,用Annexin V-FITC/PI双染色法检测在同样浓度的SeIV和SeMet培养下细胞的凋亡率,并设置不做硒处理的空白对照。用MTT法检测细胞存活率;检测细胞培养液中乳酸脱氢酶(LDH)释放率、细胞内的超氧化物歧化酶SOD活力和谷胱甘肽GSH的含量。相同浓度时(0.8μg Se/mL),与对照相比,SeIV诱导细胞凋亡显著率上升(p<0.05),SeMet变化不显著。一定浓度的SeIV(≥0.4μg Se/mL)和SeMet(≥40μg Se/mL)与对照组相比均显著降低细胞的存活率(p<0.05);随着Se含量的升高细胞的存活率呈下降趋势,SeIV、SeMet的IC_(50)分别为2.56、215.55μg Se/mL。当SeIV浓度为≥0.8μg Se/mL,SeMet浓度为≥40μg Se/mL时,细胞的LDH释放率均显著高于对照组(14.80%)(p<0.05)。当SeIV浓度≥4μg Se/mL,SeMet≥4μg Se/mL时,细胞的SOD活性均显著低于对照组(56.76 U/mg prot)(p<0.05)。当SeIV浓度≥4μg Se/mL,SeMet浓度为160μg Se/mL时,细胞的GSH含量均显著低于对照组(61.67μg/mg prot)(p<0.05)。一定浓度的SeIV和SeMet会使Caco-2细胞产生氧化应激而导致细胞毒性,诱导细胞凋亡。The cytotoxicity and oxidative stress which was induced by selenite(SeIV)and selenomethionine(SeMet)in Caco-2 cells was investigated.Caco-2 cells were exposed to SeIV,SeMetat the difference dose for 24 h.At the same dose of SeIV and SeMet(0.8μg Se/mL),the apoptosis of Caco-2 cells was detected by Annexin V-FITC/PI assay.The cell viability was evaluated by MTT assay.The leakage of lactate dehydrogenase(LDH),the activity of the superoxide dismutase(SOD),the glutathione(GSH)content were detected respectively.At the dose of 0.8μg Se/mL,SeIV increased the apoptosis of Caco-2 cells(p<0.05),but there was no change in SeMet treatment.SeIV(≥0.4μg Se/mL),SeMet(≥40μg Se/mL)could all decrease the cell viability in a certain dose range(p<0.05)and as their exposure concentration increased,Caco-2 cell viability was gradually decreased.The IC50 values of Caco-2 cell exposed to SeIV and SeMet for 24 h were determined to be 2.56,215.55μg Se/mL,respectively.SeIV≥0.8μg Se/mL and SeMet≥40μg Se/mL showed the higher leakage of LDH than the control group 14.80%(p<0.05).SeIV≥4μg Se/mL and SeMet≥4μg Se/mL could decreased the SOD activity(56.76±3.64 U/mg prot)(p<0.05).SeIV≥4μg Se/mL and SeMet(160μg Se/mL)could decreased the GSH contents at the same time(61.67μg/mg prot)(p<0.05).A certain concentration of SeIV and SeMet can induce oxidative stress in Caco-2 cells,leading to cytotoxicity and inducing apoptosis.

关 键 词：亚硒酸钠 硒代蛋氨酸 CACO-2细胞 氧化应激 细胞凋亡 细胞毒性 

分 类 号：R735.35[医药卫生—肿瘤]