芝麻素通过Wnt/β-catenin通路调控大鼠骨髓间充质干细胞成骨细胞分化预防骨质疏松的作用研究  

Sesamin regulates the differentiation of osteoblasts by rat bone marrow mesenchymal stem cells through Wnt/b-catenin pathway to prevent osteoporosis

作　　者：马忠平[1] 杨云[1] 张志峰[1] 叶楠[1] 杨毅峰 MA Zhongping;YANG Yun;ZHANG Zhifeng;YE Nan;YANG Yifeng(Department of Orthopaedics, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, China)

机构地区：[1]内蒙古医科大学第二附属医院关节外科,内蒙古呼和浩特010030

出　　处：《中国骨质疏松杂志》2021年第1期50-54,59,共6页Chinese Journal of Osteoporosis

摘　　要：目的探讨在体外环境下芝麻素对小鼠骨髓间充质干细胞向成骨细胞分化的影响。方法从大鼠股骨中提取骨髓间充质干细胞(bone marrow stromal cells,BMSCs),诱导其向成骨细胞分化。使用FH535(1μmol/L)沉默Wnt/β-catenin信号通路,建立沉默组,同时建立非沉默组。两组同时给予芝麻素(0、1或10μmol/L)干预,检测细胞增殖/毒性,同时检查碱性磷酸酶(alkaline phosphatase,ALP)、成骨相关转录因子抗体(osterix,OSX)、SRY-box 9(SOX9)、Runt相关转录因子2(recombinant runt related transcription factor 2,RUNX2)、骨钙素(osteocalcin,OCN)、b-连环蛋白(β-catenin)、低密度脂蛋白受体相关蛋白5(low density lipoprotein receptor-related protein 5,LRP5)以及糖原合成酶激酶-3b(glycogen synthase kinase-3b,gsk-3b)的表达水平。结果芝麻素对BMSCs的增殖无明显影响,且无细胞毒性作用。较高浓度的芝麻素诱导Wnt/β-catenin信号通路,增加ALP、OSX、SOX9、RUNX2和OCN的表达(P<0.05)。沉默Wnt/β-catenin后,RUNX2和OCN表达减弱。结论芝麻素有通过调节Wnt/β-catenin信号通路促进大鼠骨髓间充质干细胞向成骨细胞分化的趋势,对重塑大鼠骨结构有一定影响。芝麻素对骨质疏松有治疗和预防作用。Objective To investigate the effect of sesamin on osteoblast differentiation in rat bone marrow stromal cells in vitro.Methods Bone marrow mesenchymal stem cells(BMSCs)were extracted from rat femurs and induced to differentiate into osteoblasts.The Wnt/b-catenin signal pathway was silenced by FH535(1μmol/L).The silent group was established,and the non-silent group was established at the same time.Both groups were intervened with sesamin(0,1 or 10μmol/L)at the same time.Cell proliferation/toxicity and alkaline phosphatase(ALP),osteoblast related transcription factor antibodies(OSX),SRY-box 9(SOX9),Runt related transcription factor 2(RUNX2,osteocalcin(OCN),b-catenin,low density lipoprotein receptor related protein 5(LRP5),and glycogen synthase kinase 3b(GSk-3b)were detected at the same time.Results Sesamin had no significant effect on the proliferation of BMSCs and had no cytotoxicity.Sesamin at higher concentration promoted the activity of Wnt/b-catenin signaling pathway and increased the expression of ALP,OSX,SOX9,RUNX2,and OCN.After silencing Wnt/b-catenin,the expression of RUNX2 and OCN decreased.Conclusion Sesamin has a tendency to promote rat bone marrow mesenchymal stem cells to differentiate into osteoblasts by acticating Wnt/b-catenin signal pathway,and has a certain effect on the remodeling of bone structure in rats.Sesamin has therapeutic and preventive effects on osteoporosis.

关 键 词：骨髓间充质干细胞 成骨细胞 芝麻素 WNT信号通路 骨质疏松 大鼠 

分 类 号：R-332[医药卫生]