玉米须多糖的乙醇沉淀分离及体内免疫调节作用研究  

Isolation of polysaccharide from Stigma maydis by ethanol precipitation and enhanced efficacy of immunization in vivo

作　　者：宫春宇[1,2] 刘羽婷 单佳明 徐硕 邢悦 GONG Chunyu;LIU Yuting;SHAN Jiaming;XU Shuo;XING Yue(College of Food and Bioengineering,Qiqihar University,Qiqihar 161006,China;Beverage Engineering Technology Research Center of Fruit-Vegetable and Coarse Cereals of Heilongjiang Province,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006 [2]黑龙江省果蔬杂粮饮品工程技术研究中心,黑龙江齐齐哈尔161006

出　　处：《食品与发酵工业》2021年第1期143-147,共5页Food and Fermentation Industries

基　　金：国家自然科学基金(31301596);黑龙江省教育厅基本业务专项(粮头食尾)(LTSW201702);植物性食品加工技术特色学科专项(YSTSXK201820);黑龙江省优质特色果蔬杂粮开发及产业化创新团队项目(135309112)。

摘　　要：运用乙醇沉淀分离方法,将玉米须超滤多糖(corn silk polysaccharide,CSP)分级纯化为3种多糖CSP-1、CSP-2和CSP-3,并研究纯化后多糖对小鼠体液免疫功能、细胞免疫功能和主要器官的影响。结果显示,CSP主要由不溶于体积分数80%乙醇的高分子量多糖CSP-2组成,其含量占CSP的近一半。活性研究发现,玉米须多糖CSP以及2种分级多糖CSP-1和CSP-2可以有效提高小鼠抗体产生量,提高体液免疫能力;也可以有效激活巨噬细胞,提高其吞噬能力,增强细胞免疫能力;而CSP-3仅能提高体液免疫能力。CSP、CSP-1和CSP-3对小鼠胸腺和脾的生长有促进作用。4种多糖对小鼠心脏、肾、肺、肝和脑的生长没有显著影响。组间活性比较显示,CSP-1调节体液免疫功能优于CSP-2和CSP-3,而CSP-2对细胞免疫功能的调剂作用明显优于CSP和阳性对照。By ethanol precipitation,the polysaccharide(CSP)extracted by ultrafiltration from Stigma maydis was isolated to three fractions,CSP-1,CSP-2 and CSP-3.The effect of this four kinds of polysaccharides on humoral and cellular immune functions and mainly organs in mice were studied.The results showed that CSP mainly contained CSP-2 which was precipitated by 80%ethanol.CSP,CSP-1 and CSP-2 could effectively improve antibody production and humoral immunity,activate macrophage,improve macrophage phagocytosis,but CSP-3 only could improve humoral immunity.CSP,CSP-1 and CSP-3 could increase immune organs index in mice.But all kinds of polysaccharides could not increase the heart,kidney,lung,liver and brain growing.Compared with each other,CSP-1 has a better activating effect on humoral immunity function than CSP-2 and CSP-3.CSP-2 has a better activating effect on cellular immune function than CSP and the positive control.

关 键 词：玉米须 多糖 免疫调节 乙醇沉淀 

分 类 号：R285.5[医药卫生—中药学]