水曲柳松散型愈伤组织诱导及悬浮培养体系的优化  

Induction of Loose Callus and Optimization of Suspension Culture System of Fraxinus mandshurica

作　　者：刘林 陈思齐 董宇飞 邸鑫宇 詹亚光[1] 齐凤慧[1] Liu Lin;Chen Siqi;Dong Yufei;Di Xinyu;Zhan Yaguang;Qi Fenghui(College of Life Science,Northeast Forestry University,Harbin Heilongjiang 150040,China;Fuxin Industrial Technology Research Institute,Fuxin Liaoning 123000,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]阜新市产业技术创新推广中心(阜新市产业技术研究院),辽宁阜新123000

出　　处：《西南林业大学学报（自然科学）》2021年第1期24-32,共9页Journal of Southwest Forestry University：Natural Sciences

基　　金：黑龙江省应用技术研究与开发计划项目(GA19B201)资助;林木遗传育种国家重点实验室开放课题资助;东北林业大学生命科学学院大学生创新项目资助。

摘　　要：以水曲柳组培苗的叶片和叶柄为研究材料诱导愈伤组织,通过生长量、接种量、pH值,培养基中碳、氮、磷等因素优化悬浮细胞培养体系。结果表明:以WPM为基本培养基,添加6 mg/L TDZ+5 mg/L 6-BA时,对水曲柳叶柄的愈伤组织诱导率最高,达到90.91%;在只添加6 mg/L TDZ时,叶片的愈伤组织诱导率最高为87.5%。在WPM+0.3 mg/L TDZ+1.5 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/L IBA的培养基中,可以诱导出松散型的愈伤组织,悬浮培养时细胞的生长周期为18 d。在3%的蔗糖浓度下,细胞增长量最高,一个生长周期内鲜质量增加了400%。氮磷浓度超过正常水平不利于细胞的生长。最终获得了能够长期继代培养水曲柳单细胞和小细胞团的悬浮培养体系。The leaves and petioles of Fraxinus mandshurica seedlings were used as research materials to induce callus. Then the growth, inoculation amount, pH and the concentration of carbon, nitrogen and phosphorus of the medium were optimized to establish a suspension cell culture system. It was found that the callus induction rate of petiole was up to 90.91% cultured on the WPM medium supplemented with 6 mg/L TDZ+5 mg/L 6-BA,while the rate for leaf was up to 87.5% cultured on the WPM supplemented with 6 mg/L TDZ. The loose callus was successfully induced on the the WPM medium supplemented with WPM+0.3 mg/L TDZ+1.5 mg/L6-BA+0.1 mg/L NAA+0.5 mg/L IBA. The growth period of suspension cells was 18 d. The fastest growth was found at 3% sugar, which increased 400% in fresh weight in a growth period. Higher concentrations of nitrogen and phosphorus were bad for the growth of the cells. A suspension cell culture system was established, which cells can be subcultured for generations.

关 键 词：水曲柳 愈伤组织诱导 松散型愈伤组织 细胞悬浮培养 

分 类 号：S772.37[农业科学—森林工程]