火龙果中螺螨酯残留检测及膳食摄入风险评估  

Determination of spirodiclofen in dragon fruit and risk assessment for dietary residue intake

作　　者：吴绪金 马婧玮 汪红 安莉 乔成奎[2] 马莹 谢汉忠[2] WU Xujin;MA Jingwei;WANG Hong;AN Li;QIAO Chengkui;MA Ying;XIE Hanzhong(Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Zhengzhou Fruit Research Institute,CAAS,Zhengzhou 450009,Henan,China)

机构地区：[1]河南省农业科学院农业质量标准与检测技术研究所,郑州450002 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《果树学报》2021年第1期90-98,共9页Journal of Fruit Science

基　　金：河南省科技创新杰出青年(184100510015);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ZFRI)。

摘　　要：【目的】建立螺螨酯在火龙果全果和果肉中的残留检测方法,并对火龙果果肉中的残留量进行膳食摄入评估。【方法】样品中螺螨酯加乙腈提取,N-丙基乙二胺和石墨化碳黑净化,离心,上清液过滤膜后超高效液相色谱三重四极杆串联质谱检测,外标法定量。【结果】火龙果全果和果肉中螺螨酯平均回收率分别为87%~105%和81%~90%,相对标准偏差分别为4.6%~13%和6.6%~8.1%。火龙果全果和果肉中螺螨酯的最低检测浓度(w,后同)均为0.01 mg·kg^-1。【结论】按本试验设计进行施药,不同采收间隔期火龙果全果和果肉中螺螨酯的残留量分别为<0.71 mg·kg^-1和<0.14 mg·kg^-1。螺螨酯的普通人群国家估计每日摄入量是0.003 1 mg,占日允许摄入量的0.5%左右,认为对一般人群健康不会产生不可接受的风险。【Objective】The present study developed a method to determine of spirodiclofen based on liquid chromatography triple quadrupole mass spectrometry(LC-MS/MS),which is widely used in analytical labs.We investigated the behavior of terminal residues of spirodiclofen in dragon fruit and flesh under field conditions,risk assessment for dietary intake based on supervised residue trial data,the effects of application frequency and rate and of the interval to harvest on terminal residues.【Methods】The supervised field trials were carried out in six provinces in the year of 2018.Two treatments were included in this study to determine spirodiclofen residues at high and low application rates.Each treatment had 3 replicated plots,and each plot consisted of 2 dragon trees.The low application rate was 80 mg·kg^-1 and the high application rate 120 mg·kg^-1.Both the low and high application rates were used in two or three applications(14 days intervals each).The dragon fruit and flesh were sampled at 7,14 and 21 days after the last spraying.Immediately after collecting,the samples were put into polyethylene bags and transported to the laboratory.The fruit stalk of the dragon samples collected in the field was cut off.The dragon fruit were evenly cut into 4-8 portions along the longitudinal direction.The nonadjacent fruit petals were divided into two groups.The first group was used to prepare the whole fruit samples,and the second group was used to prepare the flesh samples.All the samples were crushed with dry ice and were packed in polyethylene bags,labelled,and stored at-20℃until analyzed.10 g subsample of homogeneous dragon fruit and flesh was put into a 100 mL centrifuge tube,and 5 mL distilled water,20 mL acetonitrile(HPLC grade),and 2.5 g sodium chloride were added in turn.The sample was vortexed for 10 min at high speed on a vortexer.The mixture was centrifuged at 4000 r·min-1 for 5 min,and 2 mL of the supernatant was transferred to a 2 mL centrifuge tube containing 0.05 g PSA and 0.02 g GCB,vortexed for 1 min a

关 键 词：火龙果 螺螨酯 残留检测 膳食摄入评估 

分 类 号：S667.9[农业科学—果树学]