浓缩生长因子纤维蛋白治疗牙龈退缩疗效评价及对牙龈成纤维细胞增殖的影响  

Curative Effect of Concentrated Growth Factor Fibrin in the Treatment of Gingival Recession and its Impact on Proliferation of Human Gingival Fibroblasts

作　　者：李敏慧 江烨 杨雪超[2] LI Min-hui;JIANG Ye;YANG Xue-chao(Graduate School of Guangzhou Medical University,Longhua District People's Hospital,Guangzhou 518000,Guangdong,China;Department of Dental Pulp,Stomatological Hospital,Guangzhou Medical University,Guangzhou 510182,Guangdong,China)

机构地区：[1]广州医科大学研究生院龙华区人民医院景龙社康服务中心,广东广州518000 [2]广州医科大学附属口腔医院牙体牙髓科,广东广州510182

出　　处：《中国美容医学》2020年第12期106-108,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨浓缩生长因子纤维蛋白(Concentrated growth factor fibrin,CGF)治疗牙龈退缩的效果及对牙龈成纤维细胞(Human gingival fibroblast,HGF)增殖的影响。方法:选择2018年6月-2019年6月笔者医院收治的30例牙龈退缩患者为研究对象,采集患者静脉血制备CGF膜,将CGF膜固定于术区进行治疗,观察术前及术后6个月的牙周袋深度(Pocket depth,PD)、退缩高度(Heigh of recession,HR)、角化龈宽度(Keratinized mucosa width,KMW)、红色美学评分(Pink aesthetic score,PES)和白色美学评分(White aesthetics score,WES)。取正常牙龈组织,采用贴壁法传代培养成HGF,在电镜下观察CGF膜上HGF的黏附情况,采用CCK-8法检测HGF的增殖情况。结果:与术前比较,术后6个月患者的PD[(2.89±0.57)mm vs(2.58±0.36)mm]、KMW[(3.99±0.68)mm vs(3.55±0.64)mm]、PES[(7.42±1.63)分vs(2.80±0.59)分]、WES[(7.90±1.74)分vs(2.77±0.55)分]均明显增高(P<0.05),HR[(1.95±0.56)mm vs(2.97±0.48)mm]明显降低(P<0.05)。通过电镜能够清晰看到HGF呈层层黏附于CGF膜上;HGF的增殖检测结果显示,培养1d、4d、7d时,CGF组的OD值[(0.54±0.08)vs(0.34±0.07)]、[(0.85±0.12)vs(0.55±0.14)]、[(1.13±0.30)vs(0.66±0.18)]均明显高于对照组(P<0.05)。结论:CGF治疗牙龈退缩的临床效果及美学效果均显著,能够明显促进HGF的黏附、生长和增殖。Objective To discuss the curative effect of concentrated growth factor fibrin(CGF)in the treatment of gingival recession and its impact on proliferation of human gingival fibroblasts(HGF).Methods 30 patients with gingival recession who was taken as research objects in our hospital from June 2018 to June 2019.Venous blood was collected to prepare CGF membrane,which was fixed in the operative area for treatment.The pocket depth(PD),heigh of recession(HR),keratinized mucosa width(KMW),pink aesthetic score(PES),white aesthetics score(WES)was observed before operation and 6 months after operation.The adhesion of HGF on CGF membrane was observed under electron microscopy.The proliferation of HGF was detected by CCK-8 method.Results Compared to before operation,the PD[(2.89±0.57)mm vs(2.58±0.36)mm],KMW[(3.99±0.68)mm vs(3.55±0.64)mm],PES[(7.42±1.63)scores vs(2.80±0.59)scores],WES[(7.90±1.74)scores vs(2.77±0.55)scores]of patients were significantly increased and the HR[(1.95±0.56)mm vs(2.97±0.48)mm]of patients were significantly decreased 6 months after operation(P<0.05).By means of electron microscopy,it was clear that HGF adhered to CGF membranelayer by layer.The results of proliferation detection of HGF showed,cultured for 1d,4d and 7d,the OD value[(0.54±0.08)vs(0.34±0.07)],[(0.85±0.12)vs(0.55±0.14)],[(1.13±0.30)vs(0.66±0.18)]of CGFgroup was significantly higher than that of control group(P<0.05).Conclusion The clinical and aesthetic effects were significant of CGF in treatment of gingival recession.CGF could significantly promote the adhesion,growth,proliferation of HGF.

关 键 词：牙龈退缩 浓缩生长因子纤维蛋白 美学 牙龈成纤维细胞 增殖 

分 类 号：R781.5[医药卫生—口腔医学]