响应面法优化古尼拟青霉中活性成分提取工艺  

Optimization of Extraction Process of Free Radical Scavenging Active Components from Paecilomyces gunnii by Response Surface Methodology

作　　者：章能胜 陈晓玲 胡丰林[2] ZHANG Neng-sheng;CHEN Xiao-ling;HU Feng-lin(Department of Medicine,Anhui Health College,Chizhou 247099,China;Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽卫生健康职业学院医学系,安徽池州247099 [2]安徽农业大学安徽省微生物防治重点实验室,安徽合肥230036

出　　处：《宜春学院学报》2020年第12期5-8,共4页Journal of Yichun University

基　　金：国家自然科学基金项目(项目编号:31570024);2020年度高校优秀拔尖人才培育资助项目(项目编号:gxyq2020110);安徽省教育厅高等学校省级质量工程支持疫情防控期间高校线上教学工作特需项目(项目编号:2020zdxsjg151)。

摘　　要：为了探索古尼拟青霉RCEF4117固体培养物中清除自由基活性组分超声波提取的最佳条件,在单因素实验基础上,采用合理的实验设计方案,应用响应面法优化提取工艺。结果表明,古尼拟青霉RCEF4117固体培养物中清除自由基活性成分超声波提取最佳工艺为:提取溶剂为甲醇,提取温度为37℃,提取时间为34 min,料液比为1:30。在此条件下,实际自由基清除率为96.58%,与预测值96.61%接近。所选提取工艺合理、可行,可用于古尼拟青霉中清除自由基活性成分的提取。To optimize the conditions of ultrasonic extraction of active components of scavenging free radicals in the solid culture of the strain RCEF 4117 of Paecilomyces gunnii,the single factor tests and response surface method(RSM)were applied.The optimum extraction condition was to extract with 100%methanol at 37℃for 34 min,solid liquid ratio 1:30 mg/mL.Under these conditions,the actual free radical scavenging rate was 96.58%,which was close to the predicted value of 96.61%.The selected extraction process is reasonable and feasible,which can be used for the extraction of free radical scavenging active components from Paecilomyces gunnii.

关 键 词：古尼拟青霉 清除自由基 提取工艺 响应面法 

分 类 号：Q939.93[生物学—微生物学]