Preparation and Evaluation of MPEG-PCL Polymeric Nanoparticles Against Gastric Cancer  

作　　者：LIU Xia LI Juncan HUANG Long YANG Jin WANG Yaowen YANG Mengjia TANG Mingxiu QIU Tong 刘霞;LI Juncan;HUANG Long;YANG Jin;WANG Yaowen;YANG Mengjia;汤明秀;邱彤(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of technology,Wuhan 430070,China;China Tobacco Hubei Industrial Co.,Ltd,Wuhan 430000,China;College of Chemical and Environmental Engineering,Hubei University for Nationalities,Enshi 445000,China;State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,China;Biomedical Materials and Engineering Research Center of Hubei Province,Wuhan University of Technology,Wuhan 430070,China)

机构地区：[1]School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of technology,Wuhan 430070,China [2]China Tobacco Hubei Industrial Co.,Ltd,Wuhan 430000,China [3]College of Chemical and Environmental Engineering,Hubei University for Nationalities,Enshi 445000,China [4]State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,China [5]Biomedical Materials and Engineering Research Center of Hubei Province,Wuhan University of Technology,Wuhan 430070,China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2020年第6期1162-1168,共7页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(No.51273156);the Health Commission of Hubei Province Scientific Research Project(No.WJ2019H275)。

摘　　要：This paper provides a new treatment for gastric cancer with a new OMT delivery system.We synthesized MPEG-PCL,an amphiphilic polymer,to construct a nanoparticle encapsulated OMT by pH gradient method,and then examined the nanodrug’s therapeutic efficacy.An integral analytical method was used to characterize the structure of MPEG-PCL.The single factor method and orthogonal test were utilized to investigate the optimum preparation process.The morphology and average size of the OMT-NPs were analyzed by transmission electron microscopy and Zetasizer.CCK-8 assay and confocal fluorescent microscope were used to study the inhibitory effect on SGC-7901 gastric cancer cells.The average size of nanoparticles was 95.86±1.54 nm.The maximum encapsulation efficiency of OMT was 46.84±4.37%,while the drug loading content was 8.89±1.09%.The cumulative release of nanoparticles was 73.07±1.5%,inspected through dynamic dialysis in vitro.Compared with free OMT,OMT-NPs showed enhanced cytotoxic effects in SGC-7901 cells.The nanoparticles could efficiently deliver the OMT into the cancer cells and release it.The OMT delivery system prepared in this paper provides a potential platform for the treatment of gastric cancer..

关 键 词：MPEG-PCL NANOPARTICLES OXYMATRINE gastric cancer 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R943[医药卫生—药剂学]