A tarsus construct of a novel branched polyethylene with good elasticity for eyelid reconstruction in vivo  

作　　者：Peifang Xu Xue Feng Honghao Zheng Zhongwei Feng Zhisheng Fu Changyou Gao Juan Ye 

机构地区：[1]Department of Ophthalmology,The Second Affiliated Hospital of Zhejiang University,College of Medicine,Hangzhou,Zhejiang 310009,China [2]MOE Key Laboratory of Macromolecular Synthesis and Functionalization,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou 310027,China

出　　处：《Regenerative Biomaterials》2020年第3期259-269,共11页再生生物材料（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(2016YFC1100403);National Natural Science Foundation of China(21434006,21871233,81670888,81870635 and 81900906);Zhejiang Provincial Natural Science Foundation of China(LQ19H120006).

摘　　要：Branched polyethylene(B-PE)elastomer was investigated for its potential medical application as a tarsus construct.The in vitro results showed that the B-PE and processed B-PE films or scaffolds did not exhibit noticeable cytotoxicity to the NIH3T3 fibroblasts and human vascular endothelial cells(ECs).The B-PE scaffolds with a pore size of 280–480 mm were prepared by using a gelatin porogen-leaching method.The porous scaffolds implanted subcutaneously in rats exhibited mild inflammatory response,collagen deposition and fast fibrovascularization,suggesting their good biocompatibility.Quantitative real-time PCR analysis showed low expression of pro-inflammatory genes and up-regulated expressions of collagen deposition and vascularization-related genes,validating the results of historical evaluation in a molecular level.The B-PE scaffolds and Medpor controls were transplanted in rabbits with eyelid defects.The B-PE scaffolds exhibited a similar elastic modulus and provided desirable repair effects with mild fibrous capsulation,less eyelid deformities,and were well integrated with the fibrovascular tissue compared with the Medpor controls.

关 键 词：branched polyethylene ELASTICITY scaffolds fibrovascularization eyelid reconstruction 

分 类 号：R77[医药卫生—眼科]