姜黄素通过下调microRNA-125b保护人视网膜色素上皮细胞免受高糖诱导的细胞损伤  

Curcumin protects human retinal pigment epithelial cells from damage induced by high glucose by down-regulating microRNA-125b

作　　者：苏军华[1] 焦永伟[2] 魏宏莲[1] SU Junhua;JIAO Yongwei;WEI Honglian(Department of Laboratory,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Orthopaedics,Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang 050000)

机构地区：[1]河北医科大学第二医院检验科,石家庄050000 [2]河北省中医院骨科,石家庄050000

出　　处：《中国比较医学杂志》2020年第12期30-35,共6页Chinese Journal of Comparative Medicine

基　　金：河北省2017年度医学科学研究重点课题计划项目(20170120)。

摘　　要：目的探究姜黄素对高糖诱导的人视网膜色素上皮(RPE)细胞中microRNA-125b(miR-125b)表达及细胞损伤的影响。方法体外培养ARPE-19细胞,分为对照组、高糖组、高糖+姜黄素组、高糖+miR-125b抑制物(inhibitor)组、高糖+抑制物阴性对照(inhibitor-NC)组,MTT检测ARPE-19细胞活性,Annexin V-FITC/PI双染法检测ARPE-19细胞凋亡情况,2′-7′-二氯荧光素二乙酸酯(DCFH-DA)探针负载法检测细胞内活性氧(ROS)水平,酶联免疫吸附试验(ELISA)法检测细胞超氧化物歧化酶(SOD)水平,RT-qPCR检测细胞中miR-125b表达水平。结果MTT实验结果显示,姜黄素可显著提高高糖环境下ARPE-19细胞活性。姜黄素可显著降低高糖环境下ARPE-19细胞凋亡率及ROS生成,增加SOD表达。RT-qPCR实验结果显示,姜黄素可显著降低miR-125b表达水平。结论姜黄素可能通过下调miR-125b表达降低ROS生成及增加SOD表达,提高ARPE-19细胞抗氧化能力保护其免受高糖诱导的细胞损伤。Objective To investigate the effects of curcumin on microRNA-125b(miR-125b)expression and cell damage in high glucose-induced human retinal pigment epithelial(RPE)cells.Methods ARPE-19 cells were cultured in vitro and divided into a control group,a high glucose group,a high glucose+curcumin group,a high glucose+miR-125b inhibitor group and a high glucose+inhibitor-negative control(NC)group.MTT assays were used to detect the activity of ARPE-19 cells.Annexin V-FITC/PI double staining was used to detect apoptosis of ARPE-19 cells.A 2′-7′dichlorofluorescin diacetate(DCFH-DA)probe loading method was used to detect the level of reactive oxygen species(ROS).An enzyme-linked immunosorbent assay(ELISA)was used to detect the level of superoxide dismutase(SOD)and RT-qPCR was used to detect the expression of miR-125b in cells.Results MTT assays showed that curcumin significantly improved the activity of ARPE-19 cells in a high glucose environment.Curcumin significantly reduced the rate of ARPE-19 cell apoptosis and ROS production in a high glucose environment,and increased the expression of SOD.RT-qPCR showed that curcumin significantly reduced the expression level of miR-125b.Conclusions Curcumin may reduce ROS production and increase SOD expression by down-regulating miR-125b expression.This would improve the antioxidant capacity of ARPE-19 cells and protect them from high glucose-induced cell damage.

关 键 词：姜黄素 microRNA-125b 人视网膜色素上皮细胞 细胞损伤 高糖 

分 类 号：R-33[医药卫生]