轻度认知功能障碍患者载脂蛋白E基因、亚甲基四氢叶酸还原酶基因多态性与情景记忆能力的关系  

The study of correlation between ApoE gene and MTHFR gene polymorphism with episodic memory in mild cognition impairment patients

作　　者：于小琪[1] 陈劲龙[1] 王双艳[1] 李晴[1] 刘丰[1] YU Xiaoqi;CHEN Jinlong;WANG Shuangyan;LI Qing;LIU Feng(Department of Geriatrics,Guangzhou First People′s Hospital,Guangzhou 510180,China)

机构地区：[1]广州市第一人民医院老年病科,广州510180

出　　处：《实用医学杂志》2020年第24期3399-3403,共5页The Journal of Practical Medicine

基　　金：广州市医药卫生科技项目(编号:20181A011001);广州市产学研协同创新重大民生科技研究(编号:201704020105)。

摘　　要：目的观察轻度认知功能障碍(MCI)患者情景记忆编码和提取成绩的变化,以及这种变化与载脂蛋白E基因(ApoE基因)、亚甲基四氢叶酸还原酶基因(MTHFR基因)C677T位点多态性的关联。方法入组227例研究对象,其中MCI患者(MCI组)109例,正常对照组(NC组)118例,使用情景记忆编码及提取成绩检测方法,对两组间情景记忆编码及提取成绩和反应时间进行检测;并检测入组者ApoE基因多态性、MTHFR基因C677T位点基因多态性,分析MCI组与NC组间基因多态性分布的特点,以及两组间情景记忆成绩的关系。结果(1)MCI组编码、提取正确率均低于NC组,结果差异有统计学意义(P<0.05)。MCI组编码反应时间、提取反应时间均较NC组延长,差异有统计学意义(P<0.05)。(2)MCI组ApoEε3/ε4、ε4/ε4基因型、MTHFR C677T位点TT基因型分布频率高于NC组,差异有统计学意义(P<0.05)。(3)两组间ApoE基因、MTHFR基因C677T位点不同基因型之间情景记忆编码成绩、提取成绩差异有统计学意义(P<0.05)。结论(1)广州市老年人MCI患者的情景记忆编码能力和提取能力均有下降。(2)ApoE基因、MTHFR基因多态性是广州市老年人MCI发病的重要危险因素,ApoE基因、MTHFR基因不同基因多态性对MCI患者情景记忆编码、提取成绩有影响。Objective To investigate the changes in episodic memory encoding(EME)and retrieval per⁃formance(RP),and correlation of these changes withApoE,MTHFR gene C677T polymorphism in the patients with Mild Cognition Impairment(MCI).Methods 227 subjects were enrolled in this study,including 109 patients with MCI(MCI group)and 118 patients with normal control group(NC group).Employing EME and RP test,The scores of EME,RP and reaction time were inspected in all the subjects.Applying restriction fragment length polymorphism detected,the ApoE,MTHFR gene C677T polymorphism were detected,and its feature of its associ⁃ation with the scores of episodic memory were analyzed.Results(1)The accuracy rate of encoding,retrieval accuracy in MCI group were lower than that in NC group,the differences were statistically significant(P<0.05).The reaction time of encoding and retrieval in MCI were obviously extended compared with that in NC group,the results were significantly different(P<0.05).(2)The difference in distribution frequency of ApoEε3/ε4、ε4/ε4、MTHFR gene C677T TT genotype in MCI group were higher than that of NC group,the results were different(P<0.05).(3)The differences in score of EME and RP in different ApoE,MTHFR C677T genotypes among the two groups were statistically significant(P<0.05).Conclusions(1)The ability of encoding and retrieval of episodic memory in the elderly MCI patients of Han nationality in Guangzhou were decreased.(2)Polymorphism of ApoE gene and MTHFR gene were important risk factors of MCI in the elderly of Han nationality in Guangzhou,the gene polymorphism has an effect on encoding and retrieval of episodic memory in MCI patients.

关 键 词：轻度认知功能障碍 载脂蛋白E基因 亚甲基四氢叶酸还原酶基因 基因多态性 情景记忆 

分 类 号：R743.9[医药卫生—神经病学与精神病学]