低谷蛋白大米(W0868)对小鼠营养状况及肾功能的影响  

Effects of low-gluten rice(W0868)feeding on nutritional status and renal function in mice

作　　者：王梨名 刘金凤 陈佳[1] 罗佳[1] 汪晓月 何娅妮[1] 蔡明玉[1] WANG Liming;LIU Jinfeng;CHEN Jia;LUO Jia;WANG Xiaoyue;HE Yani;CAI Mingyu(Department of Nephrology,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042;Department of Gastroenterology,First Affiliated Hospital,Army Medical University(Third Military Medical University),Chongqing,400038,China)

机构地区：[1]陆军军医大学(第三军医大学)大坪医院肾内科,重庆400042 [2]陆军军医大学(第三军医大学)第一附属医院消化内科,重庆400038

出　　处：《第三军医大学学报》2021年第1期68-74,共7页Journal of Third Military Medical University

基　　金：重庆市技术创新与应用示范项目(CSTC2018jscx-msybX0093);国家自然科学基金青年科学基金(81800646)。

摘　　要：目的研究低谷蛋白大米(W0868)饮食对小鼠营养状况及肾功能的影响。方法雄性6~8周龄C57/BL6雄性小鼠30只,采用随机数字表法分为常规饲料组、正常大米组和低谷蛋白大米组(n=30)。常规饲料组给予常规鼠粮,普通大米组给予普通大米与常规饲料1:1混合的普通米饲料,低谷蛋白大米组给予低谷蛋白大米与常规饲料1:1混合的低谷蛋白米饲料喂养。每只小鼠单独给予自由摄食,每日定时监测摄食量。分别于饮食干预后每间隔1周监测体质量,于第4、8、12周时留取24 h尿,于12周时留取血清和肾组织标本。采用评估营养状况的指标包括体质量、血清学指标(总蛋白、白蛋白、前白蛋白、球蛋白、钙、磷等),肾功能指标包括血肌酐、尿素氮,尿液检测指标包括尿肌酐、尿微量白蛋白/尿肌酐(ACR)、β-N-乙酰氨基葡糖糖苷(NAG),24 h尿蛋白总量。肾组织病理损伤程度采用PAS染色。结果同一时间点各组每只小鼠的平均摄食量和各组小鼠体质量在饮食干预期间未见明显差异。各组小鼠血清总蛋白、白蛋白、球蛋白、钙、磷等营养状况指标及肾功能指标于饮食干预12周后未见明显差异。低谷蛋白米组小鼠尿蛋白总量于12周时低于饲料组和普通大米组,尿ACR、NAG水平在4、8、12周时均低于饲料组和普通大米组。各组小鼠肾组织病理学改变未见明显差异。结论低谷蛋白大米(W0868)饮食可以明显降低小鼠尿蛋白水平而保护肾功能,并且对小鼠营养状况无影响。Objective To observe the effects of low-gluten rice feeding on nutrient metabolism and renal function in mice.Methods Thirty male C57/BL6 mice aged 6-8 weeks were randomly divided into 3 equal groups for dietary intervention with normal chow(control group),common rice mixed at 1:1 ratio with normal chow(normal rice group),or low-gluten rice mixed at 1:1 with normal chow(low-gluten rice group).Each mouse was given free access to food separately,and their food intake was monitored on a daily basis.Body weight of the mice was measured once a week during dietary intervention.Urine samples were collected at 4,8,and 12 weeks during the feeding,and serum and renal tissue samples were obtained at 12 weeks to assess the parameters related with the nutritional status(total protein,albumin,prealbumin,globulin,calcium,and phosphorus levels),renal function(blood creatinine and urea nitrogen),and the urine markers[urine creatinine,urine albumin/urinary creatinine ratio(ACR),β-N-acetylglucosaminide(NAG),and urine protein].PAS staining was used for pathological examination of the renal tissues of the mice in the 3 groups.Results The average food intake or body weight of the mice did not differ significantly among the 3 groups during the dietary intervention.The nutritional status and the renal function at 12 weeks were similar among the 3 groups.The mice in low-gluten rice group showed a significantly lower level of total urine protein at 12 weeks and lower urine ACR and NAG levels at 4,8 and 12 weeks than those in the other two groups.None of the mice in the 3 groups showed obvious histopathological changes in the kidneys.Conclusion Low-gluten rice(W0868)diet can significantly reduce urine protein levels to protect the renal function in mice without causing adverse effect on the nutritional status.

关 键 词：低谷蛋白大米 尿蛋白 营养状况 肾功能 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R151.41[医药卫生—公共卫生与预防医学]