拮抗牛呼吸系统疾病主要病原菌乳酸菌的筛选及特性研究  

Isolation and Characterization of Lactic Acid Bacteria Antagonistic to Bovine Respiratory Diseases

作　　者：党乔 吴信豪 贾博岩[2] 刘磊[2] 马珺杨 刘树明[2] 孔令聪[2,3] 马红霞 DANG Qiao;WU Xin-hao;JIA Bo-yan;LIU Lei;MA Jun-yang;LIU Shu-ming;KONG Ling-cong;MA Hong-xia(College of Food Science and Enginerring,Jilin Agricultural University,Changchun 130118,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Animal Production and Product Quality and Safety,Ministry of Education,Changchun 130118,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]吉林农业大学动物科学技术学院,吉林长春130118 [3]动物生产及产品质量安全教育部重点实验室,吉林长春130118

出　　处：《中国兽医杂志》2020年第9期26-29,33,共5页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划(2016YFD0501301);国家自然科学基金(31702293);吉林省自然科学基金(20190201294JC)。

摘　　要：为了从发酵食品中筛选出对牛支原体、牛溶血性曼氏杆菌、牛荚膜A型多杀性巴氏杆菌有拮抗功能的乳酸菌,并进一步对其生物学特性及抑菌特性进行研究。采用牛津杯法抑菌试验筛选得到1株具有优良抑菌活性的鼠李糖乳杆菌,该菌无溶血活性,其抑菌产物具有良好的热稳定性和抗紫外照射活性,抑菌能力随pH降低而增强,经蛋白酶酶解后,抑菌活性有不同程度降低。该株乳酸菌可作为潜在益生菌株防治牛呼吸系统疾病。The aim of this study was to screen out Lactobacillus with inhibition of Mycoplasma bovis,Mannheimia haemolytica and Pasteurella multocida of bovine capsular type A from fermented food.The biological characteristics and bacteriostatic activity of the compound were also studied.The results showed that a strain of Lactobacillus rhamnosus with excellent bacteriostatic activity was screened by Oxford cup method.The bacterium had no hemolytic activity,the antimicrobial products had good thermal stability and anti-ultraviolet radiation activity.The bacteriostatic activity was increased gradually with the decreasing of pH and the bacteriostatic activity decreased after enzymatic hydrolysis.This study lays a foundation for biological control of bovine respiratory diseases.

关 键 词：乳酸菌 牛支原体 牛溶血性曼氏杆菌 牛荚膜A型多杀性巴氏杆菌 

分 类 号：Q939.117[生物学—微生物学]