Mutational Analysis of OCT4+ and OCT4− Circulating Tumour Cells by Single Cell Whole Exome Sequencing in Stage I Non-Small Cell Lung Cancer Patients  

作　　者：YAN Bo FU Shijie CHANG Yuanyuan GU Aiqin DONG Qianggang LI Rong 颜波;傅世杰;常媛媛;顾爱琴;董强刚;李榕(Department of Pulmonary Medicine,Shanghai Chest Hospital,Shanghai Jiao Tong University,Shanghai 200030,China;Department of Thoracic Surgery,Shanghai Chest Hospital,Shanghai Jiao Tong University,Shanghai 200030,China;Cancer Stem Cell Research Group,Shanghai Jiao Tong University Cancer Institute,Shanghai 200032,China)

机构地区：[1]Department of Pulmonary Medicine,Shanghai Chest Hospital,Shanghai Jiao Tong University,Shanghai 200030,China [2]Department of Thoracic Surgery,Shanghai Chest Hospital,Shanghai Jiao Tong University,Shanghai 200030,China [3]Cancer Stem Cell Research Group,Shanghai Jiao Tong University Cancer Institute,Shanghai 200032,China

出　　处：《Journal of Shanghai Jiaotong university(Science)》2021年第1期40-46,共7页上海交通大学学报（英文版）

基　　金：the National Natural Science Foundation of China(No.81773273)。

摘　　要：Circulating tumour cells(CTCs)were enriched in the peripheral blood of four patients with Stage I non-small cell lung cancer(NSCLC).Octamer-binding transcription factor-4 positive(OCT4+)and negative(OCT4−)CTCs were identified and captured by interphase fluorescence in situ hybridisation(iFISH).Single cell whole exome sequencing(WES)was performed and the corresponding bioinformatics data were analysed.OCT4+cells were successfully detected in peripheral blood collected from all four Stage I lung cancer patients.Moreover,the tumour mutational burden(TMB)values observed for OCT4+samples from the same patients were slightly smaller than those of the OCT4−samples;the difference was not statistically significant(P>0.05).Thirteen and six characteristic mutations were found in negative samples and positive samples,respectively.The findings indicate that this methodology provides a potential diagnostic index for the early detection of NSCLC.

关 键 词：non-small cell lung cancer(NSCLC) octamer-binding transcription factor-4(OCT4) circulating tumour cells(CTCs) whole exome sequencing(WES) 

分 类 号：R734.2[医药卫生—肿瘤]