高效液相色谱法同时测定新鲜烟叶中17种氨基酸含量  

Contents Determination of 17 Amino Acids in Fresh Tobacco Leaves by High Performance Liquid Chromatography

作　　者：黄丽佳 吴双凤 邓亮 梁梦洁 罗娜 王雪 郭亚东 林彦君 HUANG Lijia;WU Shuangfeng;DENG Liang;LIANG Mengjie;LUO Na;WANG Xue;GUO Yadong;LIN Yanjun(School of Pharmaceutical Science&Yunnan Key Laboratory of Pharmacology for Nature Products,Kunming Medical University,Kunming,Yunnan,China 650500)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500

出　　处：《昆明学院学报》2020年第6期26-29,共4页Journal of Kunming University

基　　金：云南省科技厅科学研究基金资助项目(2018FB150);云南中烟工业有限责任公司资助项目(2018539200370184)

摘　　要：采用高效液相色谱法测定新鲜烟叶中17种氨基酸的含量,色谱柱为SunFire C18(4.6 mm I.D.×250 mm,5μm),柱温40℃,二极管阵列检测器,检测波长254 nm,流动相流速1.0~1.5 mL/min,梯度洗脱.结果表明,17种氨基酸在2.5~50.0μg/mL范围内具有良好的线性关系(r>0.995),加标回收率在88.0%~99.6%之间,RSD为0.6%~3.5%;新鲜烟叶中含量较高的是丙氨酸、苯丙氨酸、谷氨酸和脯氨酸等,17种氨基酸的总含量达到8.87 mg/g.The contents of seventeen amino acids in fresh tobacco leaves are determined by high performance liquid chromatography(HPLC). The chromatographic column is SunFire C18(4.6 mm I.D.×250 mm,5 μm);column temperature is 40 ℃;the detection wavelength of DAD detector is 254 nm;mobile phase flow rate is 1.0~1.5 mL/min, gradient elution. The results show that seventeen amino acids have good linearity(r>0.995) in the range of 2.5~50.0 μg/mL, and the spiked recoveries are between 88.0%~99.6%,RSD:0.6%~3.5%. The higher contents of the amino acids in fresh tobacco leaves were ala, phe, glu and pro etc. The total contents of 17 kinds amino acids are 8.87 mg/g.

关 键 词：高效液相色谱 新鲜烟叶 氨基酸 含量测定 

分 类 号：R2841[医药卫生—中药学] S527[医药卫生—中医学]