miR-375靶向cyclinD2调节口腔鳞癌细胞株增殖及凋亡的研究  

Study on miR-375 regulating proliferation and apoptosis of oral squamous cell carcinoma cell lines via targeting cyclin D2

作　　者：方首镕 王晓云 蒋宁宁 孙立平 FANG Shou-rong;WANGXiao-yun;JIANG Ning-ning;SUN Li-ping(Qingdao Sanatorium of Shandong Province,Qingdao,Shandong 266000,China)

机构地区：[1]山东省青岛疗养院口腔科,山东266000 [2]徐州医科大学附属口腔医院口腔颌面外科,江苏221002 [3]济南市口腔医院口腔颌面外科,山东266000

出　　处：《中华老年口腔医学杂志》2020年第6期330-334,共5页Chinese Journal of Geriatric Dentistry

摘　　要：目的:探讨miR-375靶向细胞周期蛋白D2(cyclin D2)调节口腔鳞癌细胞株CAL27增殖及凋亡的作用。方法:培养CAL27细胞并转染阴性对照(NC)mimic、miR-375 mimic、NC siRNA、cyclinD2 siRNA、表达cyclinD的pcDNA3.1质粒及空白的pcDNA3.1质粒;检测细胞增殖活力OD490水平、细胞凋亡率、miR-375及cyclinD2的表达水平,验证miR-375靶向cyclinD2基因mRNA 3’UTR的作用。结果:转染miR-375 mimic后,CAL27细胞的OD490水平、cyclinD2表达水平、含有cyclinD2 mRNA 3’UTR的双荧光素酶报告基因的荧光活性降低,凋亡率增加(P<0.05);转染cyclinD2 siRNA后,CAL27细胞的OD490水平、cyclinD2表达水平降低,凋亡率增加;转染表达cyclinD的pcDNA3.1质粒后,miR-375 mimic降低OD490水平及cyclinD2表达水平、增加凋亡率的作用被逆转。结论:miR-375对口腔鳞癌细胞的增殖具有抑制作用、凋亡具有促进作用且靶向抑制cyclinD2是介导该作用的分子机制之一。Objective:To study the effect of mi R375 on regulating proliferation and apoptosis of oral squamous cell carcinoma cell lines CAL27 via targeting cyclinD2.Methods:CAL27 cells were cultured and transfected with negative control(NC)mimic,mi R-375 mimic,NC si RNA,cyclinD2 si RNA,cyclinD2 expressing pcDNA3.1 plasmid and blank pcDNA3.1 plasmid.Cell proliferation viable OD490 level,apoptosis rate,expression levels of mi R-375 and cyclinD2 were detected,mir-375 targeting 3’UTR of cyclin D2 m RNA was verified.Results:After transfection of mi R-375 mimic,the OD490 level,cyclinD2 expression level and the fluorescence activity of double luciferase reporter gene containing cyclinD2 m RNA 3’UTR decreased,the apoptosis rate increased(P<0.05);after transfection of cyclinD2 si RNA,the od490 level,cyclinD2 expression level decreased,and the apoptosis rate increased;after transfection of CyclinD2 expressing pcDNA3.1 plasmid,the effect of mi R-375 mimic on reducing OD490 level,cyclin D2 expression and increasing the apoptosis rate were reversed.Conclusion:mi R-375 has inhibitory effect on proliferation and promotion effect on apoptosis of oral squamous cell carcinoma cells and targeted inhibition of cyclin D2 is one of the molecular mechanisms responsible for this effect.

关 键 词：口腔鳞状细胞癌 miR-375 CYCLIND2 增殖 凋亡 靶基因 

分 类 号：R782[医药卫生—口腔医学]