TGF-β regulation of microRNA miR-497-5p and ocular lens epithelial cell mesenchymal transition  

作　　者：Jinda Wang Jingshang Zhang Ying Xiong Jing Li Xiaoxia Li Jing Zhao Guyu Zhu Hailong He Yusufu Mayinuer Xiuhua Wan 

机构地区：[1]Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital of Capital Medical University,Beijing Key Laboratory of Ophthalmology&Visual Sciences,Beijing 100005,China [2]Beijing Tongren Eye Center,Beijing Tongren Hospital of Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Sciences,Beijing 100005,China

出　　处：《Science China(Life Sciences)》2020年第12期1928-1937,共10页中国科学（生命科学英文版）

基　　金：supported by the Beijing New Star in Science and Technology(H020821380190 and Z131102000413025);the National Working Committee on Children and Women under State Council(2014108);the National Natural Science Foundation of China(30471861)。

摘　　要：The purpose of this study was to investigate the role of a human lens microRNA(miR-497-5p) in regulating epithelialmesenchymal transition(EMT) under the control of transforming growth factor beta(TGF-β). A microRNA array was used to evaluate the microRNA profiles of untreated and TGF-β-treated human lens epithelial cells in culture. This showed that TGF-βtreatment led to the upregulation of 96 microRNAs and downregulation of 39 microRNAs. Thirteen microRNAs were predicted to be involved in the pathogenesis of posterior capsule opacification(PCO). Meanwhile, overexpression of miR-497-5p suppressed cell proliferation and EMT 48 h post-transfection, and inhibition of miR-497-5p accelerated cell proliferation and EMT.Treatment with TGF-β inhibited the expression of miR-497-5p, but not cell proliferation. miR-497-5p was also found to regulate the level of CCNE1 and FGF7, which are reported to be actively involved in EMT. CCNE1 and FGF7 were bona fide targets of miR-497-5p. The results suggest that miR-497-5p participates in the direct regulation of lens epithelial cell EMTand is regulated by TGF-β. miR-497-5p may be a novel target for PCO therapy.

关 键 词：MICRORNA transforming growth factor beta(TGF-β) lens epithelial cells posterior capsule opacification 

分 类 号：R776.1[医药卫生—眼科]