Gli1抑制剂GANT61对小鼠牙胚体外发育的影响  

Effect of Gli1 Inhibitor GANT61 on Tooth Development in Vitro

作　　者：张师贤 林成泉 谢长富 陈伟辉[2] ZHANG Shixian;LIN Chengquan;XIE Changfu;CHEN Weihui(General and Emergency Department, Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University , School and Hospital of Stomatology , Fujian Medical University, Fuzhou 350001,China;Department of Oral and Maxillofacial Surgery,Union Hospital,Fujian Medical University,Fuzhou 350001,China;Department of ImplantologyⅡ,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]福建省口腔疾病研究重点实验室,福建省口腔生物材料工程技术研究中心,福建省高校口腔医学重点实验室,福建医科大学口腔医学院/附属口腔医院综合急诊科,福建福州350001 [2]福建医科大学附属协和医院口腔颌面外科,福建福州350001 [3]福建医科大学附属口腔医院种植二科,福建福州350001

出　　处：《口腔医学研究》2021年第1期23-27,共5页Journal of Oral Science Research

基　　金：福建省卫生计生科研人才培养项目(编号:2018-1-65)。

摘　　要：目的:研究外源性Gli1抑制剂GANT61对小鼠磨牙胚体外发育的影响。方法:采用小鼠磨牙胚体外培养系统,运用体视镜、组织学HE染色方式观察小鼠磨牙胚被Gli1抑制剂GANT61影响后的发育情况,并对牙胚构成部分进行测量。结果:在Gli1抑制剂GANT61作用下,实验组相对于对照组出现下磨牙牙胚发育速度减慢,成釉器凹陷且形态不规则,内釉上皮细胞呈现不规则排列,成釉器大小在高浓度GANT61组明显变小。结论:Gli1抑制剂GANT61可能通过扰乱成釉器的发育阻碍胎鼠磨牙胚的发育过程。Objective:To study the effect of Gli1 inhibitor GANT61 on tooth development in mice.Methods:The mouse molar germ in culture system was observed with stereoscope and histology after being affected by the Gli1 inhibitor GANT61,and the size of the tooth germ was measured.Results:Histological analysis showed that the degree of GANT61 treated group’s development was slower compared with that in the control group.Furthermore,the enamel organ was irregular in shape,and the inner enamel epithelial cells were irregularly arranged.The size of the enamel organ was significantly smaller in the high dose of GANT61 compared with that in the control group.Conclusion:GANT61 can block the development of molar by disturbing the development of enamel organ.

关 键 词：GLI1 GANT61 牙发育 细胞分化 

分 类 号：R73[医药卫生—肿瘤]