miR-25-3p在40例子宫内膜腺癌组织中的表达及对KLE细胞生物学功能的影响  

Expression and function of miR-25-3p in endometrial cancer

作　　者：史丽[1] 马静[1] 赵喜娃[1] 关英霞 赵连梅[1] 单保恩[1] SHI Li;MA Jing;ZHAO Xiwa;GUAN Yingxia;ZHAO Lianmei;SHAN Baoen(Department of Gynaecology and Obstetrics,Fourth Hospital of Hebei Medical University,Shijiazhuan 050011,Hebei,China)

机构地区：[1]河北医科大学第四医院妇产科,河北石家庄050011

出　　处：《山东大学学报（医学版）》2020年第12期86-91,共6页Journal of Shandong University:Health Sciences

基　　金：河北省医学科学研究重点课题计划项目(20201089)。

摘　　要：目的探讨miR-25-3p在子宫内膜腺癌组织中的表达及其对KLE细胞增殖、迁移、侵袭、凋亡的影响。方法选取2018年1月至12月在河北医科大学第四医院妇科行手术治疗的子宫内膜腺癌患者40例,采用Real-time PCR方法检测癌和癌旁正常组织中miR-25-3p的表达水平;应用细胞转染技术将miR-25-3p模拟物转染至子宫内膜腺癌细胞KLE中,转染后分为过表达组和对照组;采用Real-time PCR方法检测转染后两组KLE细胞中miR-25-3p的表达水平;分别应用流式细胞术、Transwell试验、基质胶试验及流式细胞Annexin v-PI双染实验检测两组细胞的增殖、迁移、侵袭及凋亡能力。结果miR-25-3p在40例子宫内膜腺癌和癌旁组织中的相对表达量分别为0.38±0.91、0.17±0.51,癌组织表达高于癌旁组织,差异有统计学意义(t=12.28,P=0.003);增殖实验检测结果显示,两组增殖指数分别为(59.41±0.78)%、(40.69±0.78)%,过表达组高于对照组,差异有统计学意义(各期两组间均值差异均有统计学意义:G0G1期t=12.504,P<0.001;S期t=6.902,P=0.020;G2M期t=4.203,P=0.014;PI期t=12.475,P<0.001);迁移实验检测结果显示,两组迁移细胞个数分别为(28.54±1.73)个、(19.21±1.62)个,过表达组高于对照组,差异有统计学意义(t=8.404,P=0.001);侵袭实验检测结果显示,两组的侵袭细胞个数分别为(36.66±1.53)个、(17.66±1.53)个,过表达组高于对照组,差异有统计学意义(t=15.234,P<0.001);凋亡检测结果显示,两组的细胞凋亡率分别为(2.75±0.58)%、(4.81±0.31)%,过表达组低于对照组,差异有统计学意义(t=5.443,P=0.006)。结论miR-25-3p在子宫内膜腺癌组织中呈高表达。过表达miR-25-3p可能促进子宫内膜腺癌细胞KLE的增殖、迁移和侵袭,抑制细胞的凋亡。Objective To investigate the expression of miR-25-3 p in endometrial cancer tissues,and to explore its effects on the proliferation,migration,invasion and apoptosis of KLE cells.Methods A total of 40 patients with endometrial cancer treated during Jan.and Dec.2018 were enrolled.The cancer tissues and adjacent tissues were collected,and the miR-25-3 p expressions were detected with Real-time PCR.After KLE cells were transfected with miR-25-3 p mimics,they were divided into overexpression group and control group,whose miR-25-3 p expressions were detected with Real-time PCR.The proliferation,migration,invasion and apoptosis of the two groups of KLE cells were detected with flow cytometry,Transwell assay,matrigel invasion assay,and Annexin v-PI double staining assay,respectively.Results The expression of miR-25-3 p was 0.38±0.91 in cancer tissues and 0.17±0.51 in the adjacent tissues,and the difference was statistically significant(t=12.28,P=0.003).Flow cytometry showed the proliferation index was(59.41±0.78)%in the overexpression group and(40.69±0.78)%in the control group,and the difference was statistically significant(G0 G1 phase:t=12.504,P<0.001;S phase:t=6.902,P=0.020;G2 M phase:t=4.203,P=0.014;PI phase:t=12.475,P<0.001).Transwell assay showed the number of migration cells was 28.54±1.73 in the overexpression group and 19.21±1.62 in the control group,and the difference was statistically significant(t=8.404,P=0.001).Matrigel invasion assay showed the number of invasion cells was 36.66±1.53 in the overexpression group and 17.66±1.53 in the control group,and the difference was statistically significant(t=15.234,P<0.001).Annexin v-PI double staining assay showed the apoptosis rate was(2.75±0.58)%in the overexpression group and(4.81±0.31)%in the control group,and the difference was statistically significant(t=5.443,P=0.006).Conclusion miR-25-3 p is highly expressed in endometrial cancer tissues.Overexpression of miR-25-3 p promotes the proliferation,migration and invasion but inhibits the apoptosis of KLE c

关 键 词：miR-25-3p 子宫内膜腺癌 增殖 迁移 侵袭 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]