腾冲雪鸡mtDNA D-loop区遗传多样性分析  

Genetic Diversity of mtDNA D-loop Region in Tengchong White Chicken

作　　者：王荣琼[1] 张自芳[1] 黄静 钱林东[1] 滕晓红[2] 苗永旺[2] WANG Rongqiong;ZHANG Zifang;HUANG Jing;QIAN Lindong;TENG Xiaohong;MIAO Yongwang(Department of Animal Husbandry&Veterinary Medicine,Yunnan Agricultural College of Vocational Education,Kunming 650212,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业职业技术学院畜牧兽医学院,云南昆明650212 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2021年第1期75-81,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31760659);云南省应用基础研究重点项目(2014FA032)。

摘　　要：【目的】阐明腾冲雪鸡群体遗传背景信息。【方法】采用PCR产物直接测序技术对144只腾冲雪鸡样品的mtDNA D-loop区序列变异进行检测,在此基础上结合已发表的云南其他地方鸡数据进行群体遗传学分析。【结果】在所检测的腾冲雪鸡序列中,碱基T、C、A和G平均含量分别为30.2%、29.8%、27.3%和12.6%,序列A+T平均含量为57.6%;共检测到33个核苷酸多态位点,占检测核苷酸位点总数的6.35%,其中4个为单一信息位点,29个为简约信息位点,数据突变形式主要为转换。在该群体中共发现21种单倍型,单倍型多样度(H)为0.890±0.011,核苷酸多样度(π值)为0.01641±0.00028,序列间平均核苷酸差异数和整个群体平均遗传多样性分别为8.534和0.017±0.004。系统发育分析显示:腾冲雪鸡包含A、B、E、F和G 5个母系世系,各世系所占的比例分别为25.00%、20.83%、15.28%、30.56%和8.33%。【结论】揭示腾冲雪鸡遗传多样性丰富,在世系组成比例上有别于云南其他地方鸡,且与它们有一定遗传分化。[Purpose]In order to clarify the genetic background information of Tengchong White Chicken.[Method]The sequence variation of mtDNA D-loop region of 144 samples collected from Tengchong White Chicken was detected by direct sequencing of PCR products.On this basis,combined with published data from other indigenous chickens in Yunnan,the population genetic analysis was carried out.[Results]In the sequences of Tengchong White Chicken,the average contents of T,C,A and G were 30.2%,29.8%,27.3%and 12.6%,respectively.The average A+T content of the sequences was 57.6%.A total of 33 variable sites were detected,accounting for 6.35%of the total number of nucleotides detected,of which 29 were parsimonious information sites and 4 were single information sites.The main form of data mutation was transition.A total of 21 haplotypes were found in this population.Haplotype diversity(H),nucleotide diversity(π),average nucleotide difference between sequences and mean diversity in entire population were 0.890±0.011,0.01641±0.00028,8.534 and 0.017±0.004,respectively.The phylogenetic analysis showed that Tengchong White Chicken included 5 maternal lineages A,B,E,F and G,and the proportion of each lineage was 25.00%,20.83%,15.28%,30.56%and 8.33%,respectively.[Conclution]Tengchong White Chicken is rich in genetic diversity,which is different from other Yunnan indigenous chickens in the proportion of lineage composition,and has certain genetic differentiation with them.

关 键 词：腾冲雪鸡 mtDNA D-loop 遗传多样性 母系世系 遗传分化 

分 类 号：S831.2[农业科学—畜牧学]