西方蜜蜂食用茶多酚后对3种蜜蜂报警信息素成分的触角电位反应  

Electroantennogram Response of Apis mellifera to Three Alarm Pheromone Components after Feeding on Tea Polyphenols

作　　者：王媛[1] 董坤 龚志文 李源 谭垦[2] WANG Yuan;DONG Kun;GONG Zhiwen;LI Yuan;TAN Ken(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Tropical Forest Ecology,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Science,Mengla 666303,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]中国科学院西双版纳热带植物园,热带森林生态重点实验室,云南勐腊666303

出　　处：《云南农业大学学报（自然科学版）》2021年第1期183-187,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家现代蜂产业技术体系项目(CARS-44-KXJ13)。

摘　　要：【目的】研究西方蜜蜂采集蜂食用茶多酚溶液后,对3种报警信息素成分的触角电位反应,为探讨茶多酚在神经生物学中的作用提供参考和科学依据。【方法】以西方蜜蜂为试验对象,按照长效和短效确定2个作用时间饲喂其不同质量浓度的茶多酚溶液后采用触角电位(EAG)技术检测蜜蜂对3种报警信息素乙酸异戊酯(IPA)、乙酸辛酯(OA)和乙酸苄酯(BA)的EAG反应。【结果】茶多酚长效作用比短效作用更能增强采集蜂对3种报警信息素成分的EAG反应;茶多酚短效作用的蜜蜂对OA的EAG反应比IPA小,但长效作用的蜜蜂对OA的EAG反应比IPA强;在3种报警信息素成分中,蜜蜂对BA的EAG反应最强。【结论】蜜蜂在茶多酚干预下对不同报警信息素成分的敏感性会发生明显变化,表明茶多酚可增强蜜蜂的防御能力。[Purpose]The electroantennogram(EAG)response of foragers of Apis mellifera to three alarm pheromone components was measured after they fed on tea polyphenols,in,order to provide reference and scientific basis for exploring the role of tea polyphenols in neurobiology.[Methods]Foragers of A.mellifera after feeding on different mass concentrations of tea polyphenols(TP)for different durations were used as test material.Their electroantennogram(EAG)responses to three alarm pheromone components isopentyl acetate(IPA),octayl acetate(OA)and benzyl acetate(BA)was detected by the electroantennography.[Result]Compared with short-acting,long-acting could be more effective to enhance the EAG responses of forager to three alarm pheromone components.Foragers with short-acting exhibited lower EAG response to OA than to IPA.However,the EAG response of foragers with long-acting to OA was stronger than that to IPA.BA elicited the strongest EAG response of bees among three alarm pheromone components.[Conclusion]Our results verified that the sensitivity of bees to different alarm pheromones components would change significantly under TP intervention,suggesting that TP improved the adaptability of bees to the changes of environment.

关 键 词：西方蜜蜂 茶多酚 蜜蜂报警信息素 触角电位 

分 类 号：S894.1[农业科学—特种经济动物饲养]