基于多元统计学的青稞HPLC指纹图谱研究  

Study on HPLC fingerprint of hulless barley based onmultivariate statistics

作　　者：余倩倩 殷钲皓 黄小清 熊邵文 韩泳平 YU Qian-qian;YIN Zheng-hao;HUANG Xiao-qing;XIONG Shao-wen;HAN Yong-ping(College of Pharmacy,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学药学院,四川成都610041

出　　处：《化学研究与应用》2021年第1期38-46,共9页Chemical Research and Application

基　　金：国家十二五科技支撑计划项目(2012BAI27B07)资助;研究生创新型科研项目(CX2019SZ168)资助。

摘　　要：为了筛选品质良好的青稞,选择不同产地的10批青稞样品,建立了青稞的高效液相指纹图谱,并进行了聚类分析和主成分分析,测定了儿茶素、表儿茶素和芦丁3种指标成分的含量。首先采用高效液相色谱法测得了10批青稞的色谱图,建立其共有模式,确定了11个共有峰,蓝青稞指标成分含量均高于白青稞。对共有峰数据进行聚类分析和主成分分析,10批青稞被分为两类,主成分分析得到了3个主成分,方差累积达82.266%,综合得分最高的三个样品分别为S4、S3、S1,均为西藏地区的蓝青稞,与指标成分含量测定的结果一致。指纹图谱、聚类分析结合主成分分析为青稞的指标筛选和综合评价提供了新的方法。In order to screen good quality hulless barley(Hordeum vulgare L.var.nudum Hook.f.),10 batches of hulless barley sample from different origins were selected,and high-performance liquid fingerprints of hulless barley were established.Cluster analysis and principal component analysis were performed,and contents of three index components(catechin,epicaechin and rutin)were determined.Firstly,the chromatograms of 10 batches of hulless barley were measured by high performance liquid chromatography,and a common mode was established.Eleven common peaks were determined.The content of the index components from blue hulless barley was higher than that of white hulless barley.Cluster analysis and principal component analysis were performed on the common peak data.Ten batches of hulless barley were divided into two categories.The principal component analysis obtained three principal components with a cumulative variance of 82.266%.The three samples with the highest comprehensive scores were S4,S3 and S1,which are the blue hulless barley in Tibet,and it was consistent with the results of the determination of the index component content.The combination of fingerprint analysis,clustering analysis and the principal components analysis provides a new method for index screening and comprehensive evaluation of hulless barley.

关 键 词：青稞 高效液相色谱法(HPLC) 指纹图谱 聚类分析 主成分分析 

分 类 号：O657.7[理学—分析化学]