14株北京地区犬细小病毒分离毒株的VP2、NS1基因序列分析  

Sequence Analysis of the Complete VP 2 and NS 1 Genes of 14 Canine Parvovirus Isolates in Beijing

作　　者：李少晗 由欣月 范君文 徐一 郝雲峰 崔尚金[1,2] 秦彤 LI Shaohan;YOU Xinyue;FAN Junwen;XU Yi;HAO Yunfeng;CUI Shangjin;QIN Tong(Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Scientific Observing and Experiment Station of Veterinary Drugs and Diagnostic Technology of Ministry of Agriculture,Beijing 100193,China;Beijing Animal Disease Prevention and Control Center,Beijing 102629,China;China Animal Diseases Control Center,Beijing 100125,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]兽用药物与兽医生物技术北京科学观测站,北京100193 [3]北京市动物疫病预防控制中心,北京102629 [4]中国动物疾病预防控制中心,北京100125

出　　处：《畜牧兽医学报》2021年第1期262-267,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中国农业科学院科技创新工程项目(ASTIP-IAS15)。

摘　　要：从北京地区疑似细小病毒感染的犬粪拭子中成功分离鉴定出14株犬细小病毒(CPV)毒株,并对其完整的VP2和NS1基因进行了序列分析。结果表明,鉴定到的14株CPV毒株中,7株为New CPV-2a型,7株为CPV-2c型。此外,NS1的19、33、293、588、624和656位氨基酸以及VP2的13、574位氨基酸为新鉴定的氨基酸突变位点。基于VP2、NS1基因的系统进化分析表明,大部分的New CPV-2a型和CPV-2c型毒株与广西南宁和吉林长春地区的分离毒株亲缘关系密切,说明本次分离毒株与广西或吉林地区分离毒株具有相同的起源。本研究为更好地开展犬细小病毒流行病学调查提供了有益借鉴,也为深入研究犬细小病毒变异和传播的分子机制奠定了基础。Fourteen strains of canine parvovirus(CPV)were successfully isolated and identified from feces swabs of dogs which were suspected to be infected CPV in Beijing,and their complete NS 1 and VP 2 genes were sequenced and analyzed.Among the 14 strains identified,the results showed that 7 strains were New CPV-2a and 7 strains were of CPV-2c.Besides,some new amino acid mutation sites were identified(amino acids 19,33,293,588,624 and 656 of the NS1 and amino acids 13 and 574 of the VP2).Phylogenetic analysis based on the VP 2 and NS 1 genes indicated that most of the New CPV-2a and CPV-2c strains were closely related to the isolated strains in Nanning,Guangxi,and Changchun,Jilin,indicating that the isolated strains had the same origin as the isolated strains in Guangxi or Jilin.This study provides a useful reference for better epidemiological investigation of canine parvovirus,and also builds a foundation for the in-depth study of the molecular mechanism of canine parvovirus variation and transmission.

关 键 词：犬细小病毒 分离鉴定 VP2基因 NS1基因 进化分析 

分 类 号：S852.659.2[农业科学—基础兽医学]