基于高通量测序研究锦屏极深岩层微生物多样性  

Research on microbial diversity in the Deep Strata of JinPing based on high-throughput sequencing

作　　者：李雪丹 徐绯 王璨 徐恒[1] LI Xue-Dan;XU Fei;WANG Can;XU Heng(Sichuan Key Laboratory of Soil Ecology Protection and Pollution Control,College of Life Sciences,Sichuan University,Chengdu 610064,China;School of Biotechnology and Food Science,Anyang Institute of Technology,Anyang 455000,China;Southwest Jiaotong University,Chengdu 610064,China)

机构地区：[1]四川大学生命科学学院四川省环境保护土壤生态保护与污染防治重点实验室,成都610064 [2]安阳工学院生物与食品工程学院,安阳455000 [3]西南交通大学,成都610064

出　　处：《四川大学学报（自然科学版）》2021年第1期165-172,共8页Journal of Sichuan University(Natural Science Edition)

基　　金：中央高校基本科研项目(SCU2016C002)。

摘　　要：本研究旨在探索中国锦屏地下原始极深环境中极端微生物群落结构及功能.本研究采用高通量测序技术对锦屏极深环境中4个岩石样品和7个水样中存在的微生物群落进行了研究,共获得732477条高质量序列(Clean tags),可聚类到1318个有效OTUs,各样品菌群之间存在一定差异,但其优势菌门一致,为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes).采用PICRUSt分析了样品菌群对应的基因功能,结果表明涉及碳水化合物代谢、氨基酸代谢、能量代谢、辅助因子和维生素代谢等基因功能的丰度最高.结果表明锦屏地下原始极深环境中微生物群落复杂,但主要菌群相对稳定.This study aims to explore the composition and function of the microbial community of the deep strata in Jinping.The high-throughput sequencing was used to investigate the microbial diversity and the microbial populations in the deep strata of Jinping.A total of 732,477 clean tags were obtained and clustered to 1,318 effective OTUs based on 97%sequence similarity.Though there were certain differences among the microbiota of each samples.The dominant bacteria in phylum remained the same,which are Proteobacteria,Actinobacteria,Bacteroidetes and Firmicutes.PICRUSt analysis was performed to show that abundance of the functional genes higly involved in Carbohydrate metabolism,Amino acid metabolism,Energy metabolism and Metabolism of cofactors and vitamins.The research reveals that the phylogenetic diversity of the microbial communities in the deep strata of Jinping is complex,but the main microbial communities are relatively stable.

关 键 词：微生物多样性 16s rDNA 极端环境微生物 

分 类 号：Q938[生物学—微生物学]