羊毛角蛋白在巯基乙酸胆碱中的溶解再生  

Dissolution and regeneration of wool keratin in choline thioglycolate

作　　者：袁久刚[1] 季吉 薛琪 姜哲 范雪荣[1] 高卫东[1] YUAN Jiugang;JI Ji;XUE Qi;JIANG Zhe;FAN Xuerong;GAO Weidong(Key Laboratory of Eco-Textiles (Jiangnan University), Ministry of Education, Wuxi, Jiangsu 214122, China)

机构地区：[1]生态纺织教育部重点实验室(江南大学),江苏无锡214122

出　　处：《纺织学报》2021年第1期35-39,共5页Journal of Textile Research

基　　金：国家自然科学基金项目(31771039);国家重点研发计划专项资助项目(2017YFB0309200);中央高校基本科研业务费专项资金项目(JUSRP51717A);江苏省高等学校大学生创新创业训练计划项目(201910295064Z)。

摘　　要：为提高废弃羊毛纤维的综合利用率,采用一种新型的还原性类离子液体巯基乙酸胆碱对羊毛进行溶解和再生,观察了羊毛纤维在其中的溶解过程。采用红外光谱仪、X射线衍射分析仪、差示扫描量热仪以及凝胶电泳仪对不同温度下提取的再生角蛋白性能进行了研究。结果表明:巯基乙酸胆碱能够有效打开羊毛角蛋白大分子之间的二硫键,从而实现对羊毛皮质层及鳞片层的高效溶解,120℃条件下,最高溶解质量分数可以达到16%;经巯基乙酸胆碱溶解再生制得的角蛋白,其蛋白主体结构得到保留,但是与原羊毛相比,再生角蛋白中的α-螺旋含量降低,分子质量降低,并且随着溶解温度提高,角蛋白大分子也发生了一定程度降解。In order to improve the solubility and utilization of waste wool fiber,a new type of reducing ionic liquid-choline thioglycolate,was employed for wool dissolution in the reported research.The dissolution process of a single wool fibre in choline thioglycolate was carefully observed using polarizing optical microscope.The wool keratin extracted at different temperatures was characterized through fourier transform infrared spectrometer,X-ray diffraction,differential scanning calorimetry and SDS-PAGE in detail.The results show that choline thioglycolate is able to effectively destroy the disulfide bonds in wool keratin,thus achieving efficient dissolution of the wool cortex and scales.At 120℃,the dissolution of wool in choline thioglycolate reached up to 16%,and the regenerated wool keratin retained the main body structure.However,compared with raw wool fibers,theα-helix content in the regenerated keratin and the molecular weight decreased,and the keratin macromolecules degraded seriously under higher temperature.

关 键 词：羊毛 角蛋白 巯基乙酸胆碱 离子液体 二硫键 

分 类 号：TS195.5[轻工技术与工程—纺织化学与染整工程]