重楼皂苷Ⅰ抗肝癌细胞作用的初步研究  

Preliminary study for effect of Polyphyllin I on hepatocellular carcinoma cells

作　　者：喻青青 樊旭 朱敏[1] 郗雪艳[1] 杜伯雨[1] YU Qing-Qing;FAN Xu;ZHU Min;XI Xue-Yan;DU Bo-Yu(Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院,十堰442000

出　　处：《中国免疫学杂志》2021年第1期57-60,65,共5页Chinese Journal of Immunology

基　　金：湖北省科技厅面上项目(2017CFB462);湖北医药学院研究生启动金(2018QDJZR31)资助。

摘　　要：目的:本文旨在研究重楼皂苷Ⅰ对肝癌细胞株Huh7和HepG2的抑制作用并探讨其是否具有抑制肝癌干细胞的功效。方法:不同剂量的重楼皂苷Ⅰ处理肝癌细胞后,MTT方法检测细胞存活情况;流式细胞术检测细胞凋亡情况;Transwell实验检测细胞迁移能力;磁珠分选出CD133+-Huh7和CD133--HepG2细胞后,成球实验检测重楼皂苷Ⅰ对CD133+及CD133-肝癌细胞Huh7和HepG2体外成球能力的影响。结果:重楼皂苷Ⅰ可抑制Huh7和HepG2细胞的增殖能力和迁移能力;诱导肝癌细胞发生剂量依赖性凋亡;磁珠分选和体外成球实验结果显示,重楼皂苷Ⅰ对CD133+-Huh7和CD133+-HepG2肝癌干细胞作用无显著差异,均使细胞变小,细胞形态发生皱缩。结论:重楼皂苷Ⅰ可以抑制肝癌细胞Huh7和HepG2的增殖和迁移,诱导细胞发生凋亡,并不具备明显的杀伤肝癌干细胞的能力。Objective:To study the inhibitory effect of PolyphyllinⅠon Huh7 and HepG2 hepatoma cell lines and to explore whether it has the inhibitory effect to hepatocellular carcinoma stem cells.Methods:After hepatoma cells were treated with different doses of PolyphyllinⅠ,MTT assay was used to detect cell survival,flow cytometry was used to detect cell apoptosis,Transwell was used to detect cell migration.CD133+-Huh7 and CD133+-HepG2 cells were separated by magnetic beads.The effect of Polyphyllin I on the sphere-forming ability of CD133+and CD133-hepatoma cells was detected by spheroidization test.Results:PolyphyllinⅠcould inhibit the proliferation and clone formation of Huh7 and HepG2 cells,and inhibit the migration of hepatocellular carcinoma cells.Moreover,PolyphyllinⅠcan induce dose-dependent apoptosis of hepatocellular carcinoma cells.The results of magnetic separation and pelletization in vitro showed that PolyphyllinⅠmade the cells smaller and the cell morphology shrunk,which had no difference in effect on CD133+-Huh7 and CD133+-HepG2,made cells were smaller,cell morphology shrivel.Conclusion:PolyphyllinⅠcan inhibit the proliferation,migration and induce apoptosis of Huh7 and HepG2 hepatocellular carcinoma cells,but it has no obvious the ability to kill hepatocellular carcinoma stem cells.

关 键 词：肝癌 肿瘤干细胞 重楼皂苷Ⅰ 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤]