诱变剂二甲肼对PC12细胞毒性效应及其机制研究  

Study on the Toxic Effect of the Dimethylhydrazine on PC12 Cells and its Mechanism

作　　者：史宁 郭宏举 耿帅 常李荣 SHI Ning;GUO Hong-ju;GENG Shuai;CHANG Li-rong(Strategic Support Forces Medical Center,Department of Pharmacy,Beijing 100101,China)

机构地区：[1]战略支援部队特色医学中心药学部,北京100101

出　　处：《中国药物评价》2020年第6期417-421,共5页Chinese Journal of Drug Evaluation

基　　金：战略支援部队特色医学中心医药卫生基础研究探索课题(19ZX63)。

摘　　要：目的:研究二甲肼对PC12细胞的毒性作用及其作用机制。方法:二甲肼分别以600、700、800、900、1000μmol•L^-1浓度为作用剂量,与PC12细胞孵育48 h后,采用CCK-8比色法检测二甲肼对体外培养PC12细胞增殖的抑制作用;彗星实验技术检测PC12细胞DNA的损伤作用;流式细胞术考察细胞凋亡。结果:二甲肼作用PC12细胞48 h后对其具有明显的毒性作用,细胞生长受到明显的抑制;单细胞凝胶电泳实验表明,二甲肼对体外培养PC12细胞的DNA有明显的损伤作用;流式细胞结果显示,二甲肼可诱导细胞凋亡。以上作用均具有浓度依赖性,且具有显著性差异(P<0.05)。结论:二甲肼能够显著抑制PC12细胞增殖,损伤细胞DNA,并具有剂量依赖性,其作用机制可能和细胞内过氧化有关。Objective:To study the cytotoxic effect of dimethylhydrazine on PC12 and its mechanism of action.Methods:PC12 cells was incubated in Dimethyl hydrazine in the concentration of 600,700,800,900,and 1000μmol•L^-1 for 48 h.Then detected the inhibitory effect of Dimethyl hydrazine on PC12 cells proliferation in vitro by CCK-8 colorimetric assay.Comet assay showed the DNA damage in PC12 cells.The cell apoptosis was studied by flow cytometry.Results:Dimethylhydrazine has obvious toxic effect on PC12 cells after 48h,and the cell growth is obviously inhibited.Single-cell gel electrophoresis experiments showed significant DNA damage.All above had a significant difference compared with non-treat group(P<0.05).Conclusion:Dimethylhydrazine can significantly inhibit the proliferation of PC12 cells,damage cell DNA,and is dose-dependent.Its mechanism of action may be related to intracellular peroxidation.

关 键 词：二甲肼 PC12细胞 毒性 作用机制 损伤 

分 类 号：R965.2[医药卫生—药理学]