羟喜树碱对HeLa和C33A细胞增殖、侵袭及迁移的影响  

Effects of hydroxycamptothecin on invasion and migration of HeLa and C33A cells

作　　者：赵行宇[1] 刘勃 张漠 朱志华 侯建成[1] 张巍[1] ZHAO Xing-yu;LIU Bo;ZHANG Mo;ZHU Zhi-hua;HOU Jian-cheng;ZHANG Wei(Department of Biochemistry,Jilin Medical university,Jilin Jilin 132013,China)

机构地区：[1]吉林医药学院生物化学教研室,吉林吉林132013

出　　处：《毒理学杂志》2020年第6期477-480,共4页Journal of Toxicology

基　　金：国家自然科学基金(21102055);吉林省科技厅科技发展项目(20190701062GH);吉林省教育厅“十三五”科学技术研究项目(JJKH20191062KJ)。

摘　　要：目的以不同浓度的羟喜树碱作用于宫颈癌HeLa和C33A细胞,观察羟喜树碱对两种细胞侵袭力及迁移能力的变化,比较两种细胞抑制作用的差异,并探讨其作用差异的可能途径。方法对数生长期HeLa细胞及C33A细胞,分别加入0、0.25、0.5、1.0和2.0μmol/L浓度的羟喜树碱,培养24 h。以MTS法检测细胞的增殖率,细胞划痕实验检测细胞的迁移能力,用Transwell小室法检测细胞的侵袭能力,Western blott检测MMP-2、MMP-9、E-cadherin和N-cadherin蛋白的表达。结果 MTS检测显示,羟喜树碱对HeLa及C33A细胞的增殖率均有抑制作用,且抑制作用随羟喜树碱浓度的增加而增强,差异有统计学意义(P<0.05或P<0.01);划痕实验结果显示,羟喜树碱可降低HeLa及C33A细胞的平面运动能力;Transwell侵袭小室实验结果显示,羟喜树碱对HeLa及C33A细胞的体外侵袭力具有很强的抑制作用;Western blott结果表明,羟喜树碱能够抑制HeLa及C33A细胞MMP-2、MMP-9和N-cadherin蛋白的表达,而增加E-cadherin蛋白的表达;上述抑制及增强作用,HeLa细胞均明显高于C33A细胞,差异有统计学意义(P<0.05)。结论羟喜树碱可靶向性抑制HeLa细胞的增殖,降低体外的细胞侵袭能力,其作用可能通过调控EMT相关蛋白的表达来实现。Objective To observe the inhibitory effect of different concentration hydroxycamptothecin on the invasion and migration of HPV-positive and negative cervical cancer cells, and the difference ant its possible patnway between the two cell lines are also be explored. Methods HeLa and C33 A cells were cultured, with hydroxycamptothecin at different concentration of 0, 0.25, 0.5, 1, 2 μmol/L for 24 h. The cell proliferation was detected by MTS method. The cell scratch test was used to detect the effect of hydroxycamptothecin on cell migration ability. The effect of cell invasion ability was detected by Transwell chamber. Western blot was used to detect the expressions of matrix metalloproteinase(MMP)-2, MMP-9, E-cadherin and N-cadherin. Results MTS assay showed that hydroxycamptothecin inhibited the growth of HeLa and C33 A cells, and the inhibitory effect increased dependent on drug concentration(P<0.05 or P<0.01). The result of scratch test showed hydroxycamptothecin can decrease the planar motor motility of HeLa and C33 A cells. The result of Transwell invasion chamber showed that hydroxycamptothecin has a strong inhibitory effect on HeLa and C33 A cells in vitro;Western blot result show that the expressions of MMP-2, MMP-9, and N-cadherin proteins in HeLa and C33 A cell could be inhibited by hydroxycamptothecin, while the expression of E-cadherin protein could be enhanced. The above results in HeLa cells were more significantly than that in C33 A cells. Conclusion Hydroxycamptothecin could targeted inhibit the proliferation of Hela cells and also decrease cell invasion and migration. The mechanism may be it could affect the expression of EMT-related proteins.

关 键 词：羟喜树碱 HELA细胞 C33A细胞 侵袭 迁移 

分 类 号：R737.33[医药卫生—肿瘤]