CHI3L1基因rs4950928以及GPⅥ基因rs1671153和rs1654419多态性与中国辽宁地区汉族人群大动脉粥样硬化性卒中的相关性  

Correlation between CHI3L1 gene rs4950928, GP Ⅵ gene rs1671153 and rs1654419 polymorphisms and large artery atherosclerotic stroke in Chinese Han population in Liaoning Province

作　　者：范雪 赵冬雪[2] Fan Xue;Zhao Dongxue(Department of Neurology,Bengang General Hospital,Liaoning Health Industry Group,Benxi 117000,China)

机构地区：[1]辽宁省健康产业集团本钢总医院神经内科,本溪117000 [2]中国医科大学附属盛京医院神经内科,沈阳110004

出　　处：《国际脑血管病杂志》2020年第11期827-831,共5页International Journal of Cerebrovascular Diseases

基　　金：国家自然科学基金(81301009)。

摘　　要：目的探讨辽宁地区汉族人群中几丁质酶-3样蛋白-1(chitinase 3-like 1,CHI3L1)基因rs4950928以及血小板膜糖蛋白Ⅵ(GPⅥ)基因rs1671153和rs1654419多态性与大动脉粥样硬化性卒中(large artery atherosclerotic stroke,LAA)的相关性。方法采用病例对照研究方法,病例组选取自2017年1月至2017年6月在中国医科大学附属盛京医院神经内科连续就诊的LAA患者,对照组选取自盛京医院体检中心同期年龄和性别相匹配的健康体检者。采用聚合酶链反应进行目的基因片段扩增,然后采用改良多重连接酶反应技术对rs4950928以及rs1671153和rs1654419进行基因分型。采用多变量logistic回归模型分析基因多态性与LAA发病的相关性。结果共纳入214例LAA患者和198例健康对照者。病例组CHI3L1基因rs4950928以及GPⅥ基因rs1671153和rs1654419基因型和等位基因分布频率与对照组差异均无统计学意义,显性模型和隐性模型分析差异亦无统计学意义。结论CHI3L1基因rs4950928以及GPⅥ基因rs1671153和rs1654419多态性与辽宁地区汉族人群LAA易感性无显著相关性,各等位基因的存在均无法构成LAA发病的危险因素。Objective To investigate the correlation between chitinase 3 like 1(CHI3L1)gene rs4950928,platelet membrane glycoproteinⅥ(GPⅥ)gene rs1671153 and rs1654419 polymorphisms and large artery atherosclerotic(LAA)stroke in Han population in Liaoning Province.Methods Using the case-control study method,consecutive patients with LAA were selected as the case group in the Department of Neurology,Shengjing Hospital Affiliated to China Medical University from January 2017 to June 2017,and the age-and gender-matched healthy people were selected as the control group in Shengjing Hospital Physical Examination Center during the same period.The polymerase chain reaction was used to amplify the target gene fragment,and then the modified multiple ligase reaction technology was used to genotype rs4950928,rs1671153 and rs1654419.Multivariate logistic regression model was used to analyze the correlation between the gene polymorphisms and LAA.Results A total of 214 patients with LAA and 198 healthy controls were enrolled.There was no significant difference in the distribution frequency of genotypes and alleles at the above loci between the case group and the control group,and there was also no significant difference in the dominant model and the recessive model analyses.Conclusion The CHI3L1 gene rs4950928 and GPⅥgene rs1671153 and rs1654419 polymorphisms had no significant correlation with the susceptibility of LAA in Han population in Liaoning Province.The existence of each allele cannot constitute a risk factor for LAA onset.

关 键 词：卒中 脑缺血 颅内动脉硬化 壳多糖酶3样蛋白1 血小板膜糖蛋白类 多态现象 遗传学 

分 类 号：R743.3[医药卫生—神经病学与精神病学]