不同抗性青稞品种接种BYDV-GAV后病毒含量的变化研究  

Changes of Virus Content in Different Resistant Hulless Barley Varieties Infected with Barley Yellow Dwarf Virus-GAV(BYDV-GAV)

作　　者：旺姆 杨春葆 徐齐君[1] 扎桑[1] 羊海珍 Wangmu;YANG Chun-bao;XU Qi-jun;Zhasang;YANG Hai-zhen(State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement/Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850032,China)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室/西藏自治区农牧科学院农业研究所,西藏拉萨850032

出　　处：《西藏农业科技》2020年第4期30-33,共4页Tibet Journal of Agricultural Sciences

基　　金：省部共建青稞和牦牛种质资源与遗传改良国家重点实验室自主课题(XZNKY-2019-C-007Z03);西藏自治区重大科技专项(XZ2019NA01-014)。

摘　　要：本研究以抗黄矮病青稞品系C280和感病品种康青3号为供试材料,人工接种BYDV-GAV病毒,采用TAS-ELISA方法,监测供试材料叶片和根系中的病毒含量的动态变化。研究结果表明,抗、感病材料叶片和根系中的BYDV-GAV含量均呈现出先上升后下降的变化趋势;病毒首先在新叶中被检测到,之后移动到根系中。接种10-30 d,抗病品系C280根系中的病毒含量明显低于感病品种康青3号;在测定时间内,抗、感病品种叶片中的病毒含量无明显差异。以上结果为进一步探索青稞黄矮病侵染机制和抗病机制提供依据。In the present study,the resistant BYDV-GAV virus hulless barley line C280 and susceptible cultivar Kangqing-3 were used as materials to monitor the dynamic changes of the virus content in the leaves and roots after artificially inoculated BYDV-GAV virus by the TAS-ELISA method.The results showed that the content of BYDV-GAV in leaves and roots were both increased first and then decreased in two hulless barley varieties,and the virus was primarily detected in new leaves and then moved to roots.After 10-30 days of inoculation,the virus content in the roots of disease-resistant line C280 was significantly lower than that of susceptible varieties Kangqing-3,while there was no significant difference in leaves of two varieties.The above results provided a basis for further exploring the BYDV-GAV infection and resistance mechanisms of hulless barley.

关 键 词：大麦黄矮病毒 青稞 TAS-ELISA 病毒含量 品种抗性 

分 类 号：S512.3[农业科学—作物学]