投喂虾青素对哲罗鲑虾青素含量和抗氧化力的影响  

Effects of Dietary Astaxanthin on Muscular Astaxanthin Content and Total Antioxidant Capacity in Tissues of Taimen Hucho taimen

作　　者：张立颖[1] 赵萌[1] 李文通[1] 袁丁[1] 杨贵强[1] ZHANG Liying;ZHAO Meng;LI Wentong;YUAN Ding;YANG Guiqiang(National Engineering Technology Research Center for Freshwater Fisheries,Beijing Fisheries Research Institute,Beijing 100068,China)

机构地区：[1]北京市水产科学研究所暨国家淡水渔业工程技术研究中心,北京100068

出　　处：《水产科学》2021年第1期89-95,共7页Fisheries Science

基　　金：北京市财政项目(PXM2016-179303-000037).

摘　　要：水温12~18℃,将体质量(435.63±21.32)g的哲罗鲑饲养在6.0 m×2.5 m×1.2 m的流水水泥池中,投喂含100 mg/kg人工合成虾青素(对照组)和10、20、40、60 mg/kg天然虾青素(雨生红球藻)饲料,每两周检测试验鱼肌肉中的虾青素含量和鳃、肝脏、前肠、后肠、幽门垂、肌肉、血清、血细胞的总抗氧化能力和碱性磷酸酶活性,研究人工合成虾青素和天然虾青素对肌肉中虾青素含量及其组织抗氧化力的影响。70 d的饲养结果显示:(1)与对照组相比,20 mg/kg和40 mg/kg组的试验鱼虾青素含量显著升高(P<0.05),且40 mg/kg含量组试验鱼虾青素含量显著优于20 mg/kg组(P<0.05);(2)各组试验鱼鳃、肝脏、前肠、后肠、幽门垂、血清、血细胞的总抗氧化能力显著高于对照组(P<0.05);(3)各组试验鱼鳃、后肠、幽门垂、肌肉、血清、血细胞的碱性磷酸酶活性显著高于对照组(P<0.05)。试验结果表明,与添加100 mg/kg人工合成虾青素相比,饲料中添加20~40 mg/kg天然虾青素,能显著提高哲罗鲑肌肉虾青素含量、总抗氧化能力以及碱性磷酸酶活性,建议哲罗鲑饲料中天然虾青素添加量为40 mg/kg。Taimen Hucho taimen with body weight of(435.63±21.32)g was reared in a 6 m×2.5 m×1.2 m running water cement pond and fed diets containing synthetic astaxanthin at a dose of 100 mg/kg(control group)and natural astaxanthin derived from green alga Haematococcus pluvialis at a dose of 10,20,40,and 60 mg/kg at water temperature of 12℃to 18℃,and astaxanthin content in muscle and the total antioxidant capacity and alkaline phosphatase activity in gills,liver,foregut,hindgut,pylorus,muscle,serum,and blood cells were detected in taimen once every two weeks in order to evaluate the effects of synthetic and natural astaxanthins on the muscle astaxanthin addition and the total antioxidant capacity(T-AOC)in taimen.The results showed that there was significantly higher muscular astaxanthin content in the taimen in 20 mg/kg group and 40 mg/kg group than that in the control group(P<0.05),significantly higher in 40 mg/kg group than that in 20 mg/kg group(P<0.05).The T-AOC of liver,foregut,hindgut,pyloric,serum and plasma were shown to be significantly higher in the four test groups than that in the control group(P<0.05).The alkaline phosphatase activities in gill,hindgut,pyloric,muscle,serum and plasma were significantly higher in the test groups than that in the control group(P<0.05),significantly higher alkaline phosphatase activity foregut,pyloric,muscle,serum and plasma in the 40 mg/kg group than those in other experimental groups(P<0.05).The findings indicate that,compared with the feed supplemented with 100 mg/kg synthetic astaxanthin,the diet supplemented with natural astaxanthin from 20 mg/kg to 40 mg/kg significantly improved the muscular astaxanthin content,and increase significantly the T-AOC of gill,liver,foregut,hindgut,pyloric,serum,plasma,and increase significantly the alkaline phosphatase activities of gill,hindgut,pyloric,muscle,serum,plasma.It is recommended that amount of natural astaxanthin from H.pluvialis be 40 mg/kg in diet of taimen.

关 键 词：哲罗鲑 虾青素 总抗氧化能力 碱性磷酸酶 

分 类 号：S965.199[农业科学—水产养殖]