EDC对牙本质即刻及老化微拉伸粘接强度的影响  

Effects of EDC on immediate and aging micro-tensile bonding strength of dentin

作　　者：沙青 李贺[1] 张红[1] 程博群 王莹 闫琳琳 邹馨颖 张志民[1] SHA Qing;LI He;ZHANG Hong;CHENG Boqun;WANG Ying;YAN Linlin;ZOU Xinying;ZHANG Zhimin(Department of Endodontics,Stomatology Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学口腔医院牙体牙髓科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2021年第1期173-179,共7页Journal of Jilin University：Medicine Edition

基　　金：吉林省财政厅科技项目(jsz2018170-2)。

摘　　要：目的:探讨添加1-(3-二甲基氨基丙基)-3-乙基碳二亚胺(EDC)后通用型粘接剂的细胞毒性、微拉伸粘接强度和断裂模式,阐明EDC添加量对通用型粘接剂粘接性能的影响。方法:将EDC添加到通用型粘接剂Single Bond Universal中,配制含不同浓度(0、0.1、0.2和0.3 mol·L^-1)EDC的实验性粘接剂。对数生长期L-929成纤维细胞分为阴性对照组及0、0.1、0.2和0.3 mol·L^-1 EDC组,并设空白对照组(培养液);阴性对照组细胞中加入培养液,不同浓度EDC组制备粘接剂膜并获取浸提液,将浸提液与细胞共培养,通过CCK-8法测定细胞相对增殖率(RGR),进行细胞毒性分级。选取新鲜拔除无龋的第三磨牙,制备微拉伸粘接试件,用万能试验机测试各组试件即刻(37℃去离子水中储存24 h)及老化(使用冷热循环机循环5000次)微拉伸粘接强度,即刻微拉伸测试实验分为对照组(不含EDC)及0.1、0.2和0.3 mol·L^-1 EDC组,老化微拉伸测试实验分为对照组(不含EDC)及0.1和0.2 mol·L^-1 EDC组,用体式显微镜观察试件断裂模式。结果:细胞毒性实验,各组细胞共培养24、48和72 h时RGR均在75%以上,细胞毒性为1级。即刻微拉伸测试实验,对照组和0.1、0.2及0.3 mol·L^-1 EDC组粘接强度分别为(30.71±4.36)、(39.41±6.72)、(26.51±9.54)和(18.55±5.37)MPa,与对照组比较,0.1 mol·L^-1 EDC组粘接强度明显升高(P<0.05),0.3 mol·L^-1 EDC组粘接强度明显降低(P<0.05);断裂模式,对照组及0.2和0.3 mol·L^-1 EDC组以界面破坏为主,0.1 mol·L^-1 EDC组以混合破坏为主。老化微拉伸测试实验,对照组及0.1和0.2 mol·L^-1 EDC组粘接强度分别为(21.42±1.58)、(35.70±1.17)和(16.24±3.27)MPa,与对照组比较,0.1 mol·L^-1 EDC组粘接强度明显升高(P<0.05),0.2 mol·L^-1 EDC组粘接强度明显降低(P<0.05);断裂模式,对照组和0.2 mol·L^-1 EDC组均以界面破坏为主,0.1 mol·L^-1 EDC组以混合破坏为主。结论:EDC添加到通用型粘接剂Single Bond UniversalObjective:To explore the the cytotoxicity,micro-tensile bond strength,and fracture mode of the universal adhesive after added with 1-ethyl-3(3-dimethylaminopropyl)carbodiimide(EDC),and to clarify the effect of EDC addition amount on the bonding performance of universal adhesive.Method:EDC was added to the universal adhesive Single Bond Universal to configure the experimental adhesives containing different concentrations(0.1,0.2 and 0.3 mol·L^-1)of EDC.The L-929 fibroblasts in the logarithmic phase were divided into negative control group and 0,0.1,0.2 and 0.3 mol·L^-1 EDC groups,and blank control group(culture medium)was set up;the L-929 cells in negative control were added with culture solution,and the adhesive film was prepared and the extract was obtained in different concentrations of EDC groups.The extract was co-cultured with the cells.The relative growth rate(RGR)was measured by CCK-8 method,and the cytotoxicity was graded.The freshly extracted third molars without caries were selected to prepare the micro-tensile bonding specimens.The universal testing machine was used to test the immediate(the specimens were stored in 37℃deionized water for 24 h)and aging(the specimens were treated in the hot and cold cycle machine for 5000 times of circulation)micro-tensile bonding strengths of the specimens in various groups.The immediate micro-tensile test included control group(without EDC)and 0.1,0.2 and 0.3 mol·L^-1 EDC groups,and the aging micro-tensile test included control group(without EDC),and 0.1 and 0.2 mol·L^-1 EDC groups.The fracture mode of the specimens was observed with a stereo microscope.Results:In the cytotoxicity experiment,the RGR of cells in various group at 24,48 and 72 h after co-cultivation were all above 75%,and the cytotoxicity was level 1.In the immediate micro-tensile test,the bonding strengths in control group and 0.1,0.2 and 0.3 mol·L^-1 EDC groups were(30.71±4.36),(39.41±6.72),(26.51±9.54)and(18.55±5.37)MPa.Compared with contol group,the bonding strength in 0.1 mol·L^-1 EDC g

关 键 词：碳二亚胺 细胞毒性 微拉伸粘接强度 冷热循环 

分 类 号：R783.1[医药卫生—口腔医学]