香菇单单杂交过程中核迁移和细胞质遗传规律  

Nuclear Migration and Cytoplasmic Inheritance during Mon-mon Crossing of Lentinula edodes

作　　者：宋晓霞[1] 章炉军[1] 赵妍[1] 宋春艳[1] 李传华[1] 陈明杰[1] 谭琦[1] 黄建春[1] SONG Xiaoxia;ZHANG Lujun;ZHAO Yan;SONG Chunyan;Li Chuanhua;CHEN Mingjie;TAN Qi;HUANG Jianchun(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences/Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China/National Engineering Research Center of Edible Fungi/National R&DCenter for Edible Fungi Processing/Key Laboratory of Agricultural Genetics and Breeding of Shanghai Shanghai201403 China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发中心,上海市农业遗传育种重点开放实验室,上海201403

出　　处：《食用菌学报》2021年第1期16-21,共6页Acta Edulis Fungi

基　　金：国家现代农业产业技术体系(CARS-20)。

摘　　要：通过香菇(Lentinula edodes)L808P65、EFISAAS5053P1和YAASM363P1原生质体单核菌丝体两两单单杂交,观察杂交过程中的菌落形态变化,利用ITS和rrnL部分序列分别鉴定双核菌丝体的细胞核和细胞质来源,以明确单单杂交过程中核迁移路径和细胞质遗传规律。结果表明:双亲菌落接触部位存在一条明显比周围菌丝体稀疏的分界线,3个亲本菌落的扩大速度从快到慢依次为EFISAAS5053P1、L808P65和YAASM363P1,双亲菌落接触区域的分界线均向菌落扩大速度慢的亲本菌落弯曲,有的甚至在分界线两端生长出两个与亲本菌落存在分界线的扇形菌落;细胞核迁移起始于分界线,双亲的细胞核互相向对方菌落迁移,不同培养皿中核迁移速度最快的区域不同;除位于分界线上的双核菌丝体外,左边亲本菌落的双核菌丝体细胞质来源于左边亲本菌落,右边亲本菌落的双核菌丝体细胞质来源于右边亲本菌落。Nuclear migration path and cytoplasmic inheritance during mon-mon crossing of Lentinula edodes were studied based on paired mon-mon crossings among three protoplast monokaryotic mycelia,L808 P65,EFISAAS5053 P1 and YAASM363 P1.Changes in colony morphology were observed and nuclear and cytoplasmic sources of dikaryotic mycelia were identified by ITS and partial rrnL sequences,respectively.The results showed that there was a clear demarcation line where two parent colonies contacted.Expansion speeds of three parent colonies from fast to slow were EFISAAS5053 P1,L808 P65 and YAASM363 P1 in order.Demarcation lines bent toward the parent colony with a relatively slower expanding speed and there were even two sectored colonies at both ends of a demarcation line.Nuclear migration began at a demarcation line with nuclei of two parent colonies migrated to each other.The fastest nuclear migration happened in different zones of different plates.Except dikaryotic mycelia developed on a demarcation line,dikaryotic mycelia on the left inherited cytoplasm from the parent on the left and dikaryotic mycelia on the right inherited cytoplasm from the parent on the right.

关 键 词：香菇 单单杂交 细胞核遗传 细胞质遗传 

分 类 号：S646.12[农业科学—蔬菜学]