小分子RNA干扰TLR4基因表达对宫颈癌细胞体外增殖的影响  

Effect of TLR4 gene expression interfered by small molecular RNA on proliferation of cervical cancer cells in vitro

作　　者：宋晓霞[1] 刘玉玲[2] 胡颜霞[1] 寿纪霞[1] Song Xiaoxia;Liu Yuling;Hu Yanxia;Shou Jixia(Department of Gynecology and Obstetrics,Zhengzhou People's Hospital,Zhengzhou 450053,China;Department of Gynecology and Obstetrics,Second Hospital,Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州人民医院妇产科,450053 [2]郑州大学第二附属医院妇产科,450000

出　　处：《国际医药卫生导报》2021年第1期39-41,共3页International Medicine and Health Guidance News

基　　金：河南省科技攻关项目(162102310233)。

摘　　要：目的RNA干扰技术靶向敲除TLR4基因对子宫颈癌Siha细胞生物学行为影响。方法试验分为重组载体组、空载体组与空白组3组,利用RT-PCR技术对3组细胞中的TLR4 mRNA表达情况进行检测,分别在MMT实验以及细胞划痕实验中对细胞的增殖情况进行观察并绘制其增长曲线。结果(1)重组载体组细胞中TLR4 mRNA转染前的表达水平为(81.6±2.1)%,显著高于转染后的表达水平(23.6±1.8)%,差异有统计学意义(P<0.05);空载体组与空白组中的TLR4 mRNA增殖情况在转染前后对比,差异无统计学意义(P>0.05)。(2)转染72 h时,重组载体组吸光度A值为(0.12±0.02),空载体组吸光度A值为(0.15±0.01),空白组吸光度A值为(0.16±0.02),空载体组与空白组的细胞增殖速度在转染72 h时均快于重组载体组,差异均统计学意义(均P<0.05)。(3)重组载体组细胞在划痕后培养细胞的48 h与72 h时的迁移率均慢于未转染组,差异均有统计学意义(均P<0.05)。结论小分子RNA靶向敲除TLR4基因可有效抑制子宫颈癌Siha细胞的生长速度。Objective To investigate the effect of RNA interference on the biological behavior of Siha cells in cervical cancer by targeting TLR4 gene knockout.Methods There were three groups in the experiment:a recombinant vector group,an empty vector group,and a blank group.The TLR4 mRNA expressions in the three groups were detected by RT-PCR technology.Cell proliferation was observed in MMT experiment and cell scratch experiment,respectively,and its growth curve was drawn.Results(1)The expression level of TLR4 mRNA in the recombinant vector group was significantly higher before than after transfection[(81.6±2.1)%vs.(23.6±1.8)%,P<0.05].There were no statistical differences in the TLR4 mRNA proliferation in the empty vector group and the blank group between before and after transfection(P>0.05).(2)Seventy-two hours after transfection,the absorbance value was(0.12±0.02)in the recombinant vector group,was(0.15±0.01)in the empty vector group,and was(0.16±0.02)in the blank group.The cell proliferation rates of the empty vector group and the blank control group were faster than that of the recombinant vector group 72 h after transfection(both P<0.05).(3)The cell mobilities of the recombinant vector group 48 and 72 h after scratch culture were slower than that of the non-transfected group(both P<0.05).Conclusion TLR4 gene knockout by small molecular RNA can effectively inhibit the growth of Siha cells in cervical cancer.

关 键 词：宫颈癌 RNA 小分子干扰 TLR4基因 转染 

分 类 号：R737.33[医药卫生—肿瘤]