三阴性乳腺癌中β-肾上腺素能受体的表达及与上皮间质化相关性研究  

The Expression ofβ-Adrenergic Receptors in Triple-Negative BreastCancer and Its Correlation with Epithelial Mesenchymalization

作　　者：王敏[1] 郭梅艳[2] 董雨[1] 苗玲 王友明[2] 吕晶 WANG Min;GUO Meiyan;MIAO Ling;WANG Youming;LYU Jing(Department of Thoracic Surgery,Central Hospital of Handan,Handan 056001,China;Department of Thoracic Surgery,Affiliated Hospital of Hebei Engineering University,Handan 056002,China;Medical college,Hebei University of Engineering,Handan 056038,China)

机构地区：[1]邯郸市中心医院胸外科,河北邯郸056001 [2]河北工程大学附属医院,河北邯郸056002 [3]河北工程大学医学院,河北邯郸056038

出　　处：《标记免疫分析与临床》2020年第12期2102-2106,2121,共6页Labeled Immunoassays and Clinical Medicine

基　　金：邯郸市科技局专项计划一般项目(编号:19422083008-65)。

摘　　要：目的探究三阴性乳腺癌(TNBC)中β-肾上腺素能受体(β-ARs)的表达及与上皮间质化(EMT)的关系。方法72例TNBC患者组织标本选自河北工程大学附属医院。通过免疫组织化学法对肿瘤切片进行染色以评估β-ARs的表达。通过Western印迹检测EMT标记蛋白E-钙黏蛋白和波形蛋白的表达。分别通过χ2检验及Spearman秩相关分析确定β-ARs表达与TNBC临床病理特征的相关性以及β-ARs表达与EMT标志物的关系。结果与对照组织中的表达水平相比,TNBC组织中的β1-AR和β2-AR而非β3-AR的表达显著增加(P<0.05),且β1-AR和β2-AR的表达与TNM分期和LN转移显著相关(P<0.05)。在TNBC组织中,E-钙黏蛋白表达显著降低,而波形蛋白表达显著升高(P<0.05)。高β2-AR组织中E-钙黏蛋白的表达明显低于低β2-AR组织(P<0.05)。高表达β1-AR和β2-AR的组织中,波形蛋白的表达显著增加(P<0.05)。TNBC组织中β2-AR和E-钙黏蛋白之间呈显著负相关,而β1-AR和β2-AR表达与波形蛋白水平呈正相关。结论β1-AR和β2-AR在TNBC中过表达,可能通过调节EMT过程促进TNBC的发生和发展。Objective To investigate the expressions ofβ-adrenergic receptors(β-ARs)and epithelial-mesenchymal transition(EMT)and their relationship in triple-negative breast cancer(TNBC).Methods A total of 72 TNBC patients from the Affiliated Hospital of Hebei University of Engineering were enrolled in this study.Tumor sections from all patients were stained by immunohistochemistry for assessingβ-ARs expression.The expression of EMT marker proteins including E-cadherin and Vimentin were detected by western blotting.The correlation ofβ-ARs expression with TNBC clinicopathologic features and the relationship between the expression ofβ-ARs and EMT markers were analysed.Results The expressions ofβ1-AR andβ2-AR notβ3-AR were significantly increased in TNBC tissues compared with the control tissues(P<0.05),and theβ1-AR andβ2-AR expressions were significantly correlated with TNM stage and LN metastasis(P<0.05).E-cadherin expression was significantly decreased but vimentin expression significantly increased in TNBC tissues(P<0.05).Furthermore,the expression of E-cadherin was significantly decreased in highβ2-AR than in lowβ2-AR expressing tissues(P<0.05).However,the vimentin protein expression in highβ1-AR andβ2-AR expressing tissues were significant increased(P<0.05).There was a significantly negative correlation betweenβ2-AR and E-cadherin and positive correlation between theβ1-AR andβ2-AR expression and vimentin protein levels in TNBC tissues.Conclusion The overexpression ofβ1-AR andβ2-AR contributes to the occurrence and development of TNBC by regulating EMT.

关 键 词：三阴性乳腺癌 Β-肾上腺素能受体 上皮间质化 免疫组织化学 蛋白印记法 

分 类 号：R737.9[医药卫生—肿瘤]