HPLC法对不同来源北苍术中苍术素的含量测定  

Determination of atractylodes in Rhizoma Atractylodis originating from different sources by HPLC method

作　　者：孙辑凯[1] 张梅娟[2] 张宏莲[1] 李宏铃[1] 董巍[1] SUN Jikai;ZHANG Meijuan;ZHANG Honglian;LI Hongling;DONG Wei(College of Pharmacy,Qiqihar Medical University,Heilongjiang Qiqihaer 161006;College of Life Science,Qiqihaer University,Heilongjiang Qiqihaer 161000,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学生命科学院,黑龙江齐齐哈尔161000

出　　处：《新疆医科大学学报》2021年第1期102-104,共3页Journal of Xinjiang Medical University

基　　金：黑龙江省教育厅基本科研业务项目(2016-KYYWF-0861)。

摘　　要：目的采用HPLC法测定不同产地和不同生长年份北苍术[Atractylodes chinensis(DC.)Koidz.]中苍术素的含量。方法收集不同来源的北苍术样品,以甲醇为溶剂,采用超声提取法提取,采用HPLC法测定苍术素的含量,选用Diamonsil C18(250 mm×4.6 mm,5μL)色谱柱,流动相为甲醇-水(79∶21),检测波长为340 nm,流速1 mL/min,柱温25℃,进样量20μL。结果苍术素的线性范围为0.1875~0.6000 mg/mL,方法精密度、重复性、稳定性良好;加样回收率为95.4%。不同产地北苍术中苍术素的含量为0.3232%~0.5959%,阿荣旗产北苍术饮片中苍术素含量最高。3年生、4年生、5年生苍术中苍术素的含量分别为:(0.3500±0.0048)%、(0.4550±0.0030)%、(0.3350±0.0118)%。结论本方法简便、快速、准确,适用于北苍术中苍术素的含量测定。Objective To assay atractydin content in Atractylodes chinensis(DC.)Koidz.from different sources by HPLC method.Methods Atractylodes lancea were collected from different origins,and ultrasonic extraction was used to extract atractydin by methanol as the solvent.Then atractydin was determined by HPLC.A Diamonsil C18(250 mm×4.6 mm,5μL)column was used with the column temperature at 25℃.The mobile phase consisted of methanol(A)-water(B)with gradient elution at the flow rate of 1.0 mL/min.The detection wavelength was 221 nm and the injection volume was 20μL.Results The linear range of atractylodin was 0.1875-0.600 mg/mL,the method had good precision,repeatability and stability,and the recovery was 95.4%.The content of atractylodin was 0.3232%-0.5959%in Atractylodes lancea from different producing areas.The content of atractylodin in Atractylodes lancea from Arong Banner was the highest.The contents of atractylodin in 3,4 and 5 year-old Atractylodes lancea were(0.3500±0.0048)%,(0.4550±0.0030)%,and(0.3350±0.0118)%respectively.Conclusion HPLC method was simple,quickly and accurate,which was suitable for the quantitative analysis of atractylodes in Atractylodes chinensis(DC.)Koidz.

关 键 词：北苍术 含量测定 苍术素 HPLC法 

分 类 号：R914[医药卫生—药物化学]