Urolithin A在肺癌细胞系A549中的抗增殖效应  

Anti-proliferation effect of Urolithin A in lung cancer cell line A549

作　　者：程峰[1] 窦晋涛 张庸 王祥 吴志浩[1,5] CHENG Feng;DOU Jintao;ZHANG Yong;WANG Xiang;WU Zhihao(Tumor Microenvironment Research Laboratory,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院肿瘤微环境研究室,安徽芜湖241002 [2]皖南医学院麻醉学院,安徽芜湖241002 [3]皖南医学院临床医学院,安徽芜湖241002 [4]皖南医学院检验学院,安徽芜湖241002 [5]皖南医学院基础医学院,安徽芜湖241002

出　　处：《皖南医学院学报》2021年第1期1-3,17,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(81872371);安徽省自然科学基金项目(1708085MH203);分子肿瘤学国家重点实验室开放课题(SKL-KF-2019-11)。

摘　　要：目的:研究尿石素A(Urolithin A)对肺癌细胞系A549增殖的影响和作用机制。方法:流式细胞术检测Urolithin A对A549细胞凋亡的影响;SA-β-gal检测Urolithin A对A549细胞衰老的状态;Western blot检测Cleaved PARP、p21、PUMA、p53蛋白的表达量。MTT实验探究Urolithin A对A549细胞的生长抑制作用。结果:不同浓度Urolithin A作用A549细胞,细胞凋亡率升高(P<0.05);SA-β-gal实验显示,Urolithin A促进A549细胞衰老(P<0.05);Western blot显示,Cleaved PARP、p21、PUMA、p53的蛋白表达量随Urolithin A浓度的升高而上调;不同浓度Urolithin A处理A549细胞,细胞活力以浓度依赖性下降,增殖抑制率升高(P<0.05),敲低p53能减弱Urolithin A对A549细胞引起的增殖抑制(P<0.05)。结论:Urolithin A抑制A549细胞的增殖,可能是由p53介导的。Objective:To investigate the effect and mechanism of Urolithin A on the proliferation of lung cancer cell line A549.Methods:Flow cytometry was performed to measure the effect of Urolithin A on A549 cell apoptosis,and SA-β-gal was used to detect the aging state of A549 cell.Western blot was conducted to detect the expression of Cleaved PARP,p21,PUMA and p53 protein.MTT assay was performed to investigate the growth inhibition of Urolithin A on A549 cells.Results:Urolithin A in different concentration resulted in increased apoptosis rate of A549 cells(P<0.05),and SA-β-gal revealed that Urolithin A promoted aging of A549 cells(P<0.05).Western blot showed that the protein expression of Cleaved PARP,p21,PUMA and p53 was up-regulated with added Urolithin A concentration.The activity of A549 cells treated with different Urolithin A dose was decreased in a concentration dependent manner,and the proliferation inhibition rate was increased(P<0.05),yet knockdown of p53 significantly reduced the proliferation inhibition of A549 cells induced by Urolithin A(P<0.05).Conclusion:Urolithin A can inhibit proliferation of A549 cells,which may be mediated by p53.

关 键 词：尿石素A 肺癌 细胞增殖 P53蛋白 

分 类 号：R734.2[医药卫生—肿瘤] R393[医药卫生—临床医学]