Oligo(p-phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate biomaterials  

作　　者：Nan Dai Hao Zhao Wen Yu Fengting Lv Libing Liu Shu Wang 

机构地区：[1]Bejing National Laboratory for Molecular Sciences,Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]College of Chemistry,University of Chinese Academy of Sciences.Beijing 100049,China

出　　处：《Science China Chemistry》2021年第1期143-150,共8页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China(21533012,21661132006)。

摘　　要：Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclopentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gramnegative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of intracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.

关 键 词：microbial synthesis rhodium complex NADPH PHB 

分 类 号：O643.36[理学—物理化学]