Molluscicidal effectiveness of Luo-Wei,a novel plant-derived molluscicide,against Oncomelania hupensis,Biomphalaria alexandrina and Bulinus truncatus  

作　　者：Tie-Wu Jia Wei Wang Le-Ping Sun Shan Lv Kun Yang Neng-Min Zhang Xi-Bao Huang Jian-Bing Liu Han-Cheng Liu Rui-Hua Liu Fathia A.Gawish Mohamed R.Habib Mohamed A.El-Emam Charles H.King Xiao-Nong Zhou 

机构地区：[1]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,China [2]Chinese Center for Tropical Diseases Research,Shanghai 200025,China [3]WHO Collaborating Centre for Tropical Diseases,Shanghai 200025,China [4]National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Shanghai 200025,China [5]Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China [6]Communicable Diseases Cluster,World Health Organization Regional Office for Africa(WHO/AFRO),PO Box 06,Brazzaville,Congo [7]Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention,Jiangsu Provincial Key Laboratory on Parasites and Vector Control Technology,Jiangsu Institute of Parasitic Diseases,Wuxi 214064,China [8]Hubei Jinhaichao Science&Technology Co.,Ltd,Wuhan 430206,China [9]Hubei Provincial Center for Disease Control and Prevention,Wuhan 430079,China [10]School of Chemistry and Chemical Engineering,Wuhan Textile University,Wuhan 430200,China [11]Department of Medical Malacology,Theodor Bilharz Research Institute(TBRI),Imbaba,Giza 12411,Egypt [12]Center for Global Health and Diseases,Case Western Reserve University,Cleveland,OH,USA [13]Schistosomiasis Consortium for Operational Research and Evaluation,University of Georgia,Athens,GA,USA

出　　处：《Infectious Diseases of Poverty》2019年第2期70-79,共10页贫困所致传染病（英文）

基　　金：the National Science and Technology Pillar Program(grant No.2009BAI78B07 and 2009BAI78B04);National S&T Major Program(grant No.2012ZX10004-220);Shanghai Science and Technology Committee(grant No.11XD1405400);Jjiangsu Provincial Health and Family Planning Commission(grant No.QNRC2016621 and H2018097);Wuxi Municipal Bureau of Science and Technology(grant No.CSE31N1730);Bill&Melinda Gates Foundation.

摘　　要：Background:Control of snail intermediate hosts has been proved to be a fast and efficient approach for interrupting the transmission of schistosomiasis.Some plant extracts have shown obvious molluscicidal activity,and a new compound Luo-Wei,also named tea-seed distilled saponin(TDS),was developed based on the saponins extracted from Camellia oleifera seeds.We aimed to test the molluscicidal activity of 4%TDS against the intermediate host snails in China and Egypt,and evaluate its environmental safety to non-target organisms.Methods:In the laboratory,Oncomelania hupensis,Biomphalaria alexandrina and Bulinus truncatus were exposed to 4%TDS,and the median lethal concentration(LC5o)was estimated at 24,48 and 72 h.In the field,snail mortalities were assessed 1,2,3 and 7 d post-immersion with 2.5 g/m34%TDS and 1,3,7 and 15 d post-spraying with 5 g/m24%TDS.in addition,the acute toxicity of 4%TDS to Japanese quail(Coturnixjaponica),zebrafish(Brachydanio rerio)and freshwater shrimp(Macrobrachium nipponense)was assessed by estimations of LC5o or median lethal dose(LD5o).Results:In the laboratory,the LC5o values of 4%TDS for O.hupensis were 0.701,0.371 and 0.33 mg/L at 24,48 and 72 h,respectively,and 4%TDS showed a 0.33 mg/L 24 h LC5o against B.alexandrina,and a 1.396 mg/L 24 h LCs0 against B.truncatus.Across all study regions,the pooled mortalities of O.hupensis were 72,86,94 and 98%at 1,2,3 and 7 d,following field immersion of 4%TDS at a dose of 2.5 g/m3,and were 69,77,85 and 88%at 1,3,7 and 15 d,following field spraying at 5 g/m2,respectively.4%TDS had moderate toxicity to Japanese quail(7 d LD5o>60 mg/kg)and to shrimp(96 h LCs0=6.28 mg/L;95%CI:3.53-11.2 mg/L),whereas its toxicity to zebrafish was high(96 h LCso--0.15 mg/L;95%CI:0.14-0.17 mg/L).Conclusions:4%TDS is active against O.hupensis,B.alexandrina and B.truncatus under laboratory and field conditions,and it may be a candidate molluscicide of plant origin.

关 键 词：Schistosomiasis Luo-Wei Plant-derived MOLLUSCICIDE Oncomelania hupensis BIOMPHALARIA alexandrina BULINUS truncatus Molluscicidal activity 

分 类 号：R73[医药卫生—肿瘤]