miR-141对于宫颈癌微环境改变的研究  

Microarray Screening after Down⁃regulating MiR⁃141 in Cervical Cancer Cells

作　　者：林荣春[1] 林浩亮 彭永排[1] 谢庆生[1] 梁金晓[1] 姚婷婷[1] LIN Rong-chun;LIN Hao-liang;PENG Yong-pai;XIE Qing-sheng;LIANG Jin-xiao;YAO Ting-ting(Department of Gynecological Tumor,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120)

机构地区：[1]中山大学孙逸仙纪念医院妇科肿瘤,510289

出　　处：《岭南急诊医学杂志》2020年第6期555-559,564,共6页Lingnan Journal of Emergency Medicine

基　　金：广东省自然科学基金博士启动项目(2016A030310178);广东省医学科学技术研究基金(A2017339)。

摘　　要：目的:使用基于微阵列的基因表达谱技术探索miR-141对宫颈癌细胞基因表达的影响及参与肿瘤形成的分子机制.方法:本研究采用21329 Oligo DNA表达谱芯片检测miR-141下调的HeLa和SiHa细胞以及正常HeLa和SiHa细胞中mRNA的表达,寻找差异表达基因,并使用GO分析、KEGG通路分析以及基因集富集分析探索miR-141下调后宫颈癌细胞的功能变化.结果:在宫颈癌细胞中,miR-141下调后,芯片筛选出不同的差异表达mRNA.在miR-141下调的SiHa细胞差异表达基因的GO分析中,钙黏蛋白连接、细胞-基底连接、锚定连接均有富集.基因集富集分析的结果显示3组肿瘤相关的基因集明显富集于阴性对照的HeLa细胞,分别是TNFα-NFκB通路调控基因集、乏氧相关基因集与免疫应答基因集.结论:芯片技术可以帮助我们了解宫颈癌的发病机制,为宫颈癌的预防和治疗奠定重要的基础.Objective:Using microarray screening to examine the influence of miR⁃141 on the gene expression level and tumorigenesis molecular mechanism of cervical cancer cells.Methods:In our research,expression profile microarray with 21329 Oligo DNA were used to detect the expression of mRNAs in miR⁃141 down⁃regulated HeLa and SiHa cells and normal HeLa and SiHa cells.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway and Gene Set Enrichment Analysis(GSEA)were performed to assess the potential pathways of miR⁃141 down⁃regulated SiHa cells.Results:Microarray screened differentially expressed mRNA after down⁃regulation of miR⁃141 in cervical cancer cells.For miR⁃141 downregulated SiHa cells,GO outcome showed that cadherin,binding,focal adhesion,cell⁃substrate junction and anchoring junction were some of the most enriched terms.The result of KEGG analysis showed transcriptional misregulation in cancer was the most enriched pathway in HeLa cells.And GSEA indicated several tumor related gene sets enriched in the HeLa cells of control group,including TNFαsignaling via NF⁃κB,genes up⁃regulated in response to hypoxia,protein secretion and inflammatory response.IL⁃6 is one of the differential expression genes and the most common gene in the leading edge analysis.Conclusion:miR⁃141 may promote tumorigenesis of cervical cancer by activating TNFα⁃NFκB pathway,hypoxia pathway and IL⁃6 pathway.Expression profile microarray technology may enable us to understand the pathogenesis and lay an important foundation for the prevention and treatment of cervical cancer.

关 键 词：基因表达谱 宫颈癌 miR-141 

分 类 号：R737.33[医药卫生—肿瘤]